中国神经再生研究（英文版）2013，Vol.8Issue(4)：320-327,8.DOI:10.3969/j.issn.1673-5374.2013.04.004
Minocycline inhibits the production of the precursor form of nerve growth factor by retinal microglial cells
Minocycline inhibits the production of the precursor form of nerve growth factor by retinal microglial cells
Xiaochun Yang 1Xuanchu Duan1
作者信息
- 1. Department of Ophthalmology, Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China
- 折叠
摘要
Abstract
A rat model of acute ocular hypertension was established by enhancing the perfusion of balanced salt solution in the anterior chamber of the right eye. Minocycline (90 mg/kg) was administered intraperitoneally into rats immediately after the operation for 3 consecutive days. Immunofluorescence, western blot assay and PCR detection revealed that the expression of the precursor form of nerve growth factor, nerve growth factor and the p75 neurotrophin receptor, and the mRNA expression of nerve growth factor and the p75 neurotrophin receptor, increased after acute ocular hypertension. The number of double-labeled CD11B- and precursor form of nerve growth factor-positive cells, glial fibrillary acidic protein- and p75 neurotrophin receptor-positive cells, glial fibrillary acidic protein- and caspase-3-positive cells in the retina markedly increased after acute ocular hypertension. The above-described expression decreased after minocycline treatment. These results suggested that minocycline inhibited the increased expression of the precursor form of nerve growth factor in microglia, the p75 neurotrophin receptor in astroglia, and protected cells from apoptosis.关键词
neural regeneration/ biological factor/ precursor form of nerve growth factor/ p75 neurotrophin receptor/ minocycline/ apoptosis/ nerve growth factor/ acute ocular hypertension/ retina/ photographs-containing paper/ neuroregenerationKey words
neural regeneration/ biological factor/ precursor form of nerve growth factor/ p75 neurotrophin receptor/ minocycline/ apoptosis/ nerve growth factor/ acute ocular hypertension/ retina/ photographs-containing paper/ neuroregeneration引用本文复制引用
Xiaochun Yang,Xuanchu Duan..Minocycline inhibits the production of the precursor form of nerve growth factor by retinal microglial cells[J].中国神经再生研究（英文版）,2013,8(4):320-327,8.
