中国神经再生研究（英文版）2013，Vol.8Issue(5)：410-419,10.DOI:10.3969/j.issn.1673-5374.2013.05.004
Viability of primary cultured retinal neurons in a hyperglycemic condition
Viability of primary cultured retinal neurons in a hyperglycemic condition
摘要
Abstract
using 0.05% trypsin digestion. The cell suspension was incubated in Dulbecco's modified Eagle's 79.86% of primary cultured retinal cells were positive and immunocytochemical staining showed that the purity of anti-neurofilament heavy chain antibody-positive cells was 71.53%, indicating that the primary culture system of rat retinal neurons was a reliable and stable cell system with neurons as the predominant cell type. The primary cultured retinal neurons were further treated with 0, 5.5, 15, 25, and 35 mM glucose for 24, 48, and 72 hours. The thiazolyl blue tetrazolium bromide test and flow cytometry showed that with increasing glucose concentration and treatment duration, the viability of retinal neurons was reduced, and apoptosis increased. In particular, 35 mM glucose exhibited the most significant effect at 72 hours. Thus, rat retinal neurons treated with 35 mM glucose for 72 hours can be used to simulate a neuronal model of diabetic retinopathy.关键词
neural regeneration/ peripheral nerve injury/ retina/ neurons/ apoptosis/ hyperglycemia model/ diabetic retinopathy/ glucose/ grants-supported paper/ photographs-containing paper/ neuroregenerationKey words
neural regeneration/ peripheral nerve injury/ retina/ neurons/ apoptosis/ hyperglycemia model/ diabetic retinopathy/ glucose/ grants-supported paper/ photographs-containing paper/ neuroregeneration引用本文复制引用
Yu Liu,Xueliang Xu,Renhong Tang,Guoping Chen,Xiang Lei,Limo Gao,Wenjie Li,Yu Chen..Viability of primary cultured retinal neurons in a hyperglycemic condition[J].中国神经再生研究（英文版）,2013,8(5):410-419,10.基金项目
This study was supported by the Department of Health of Hunan Province,No.B2009-050,and the Science and Technology Foundation of Hunan Province,No.2012FJ4077. （）
