中国神经再生研究（英文版）2013，Vol.8Issue(6)：554-560,7.DOI:10.3693/j.issn.1673-5374.2013.06.009
Hypoxia regulates reactive oxygen species levels in SHG-44 glioma cells
Hypoxia regulates reactive oxygen species levels in SHG-44 glioma cells
Haitao Jiang 1Jiangtao Xie 2Gaofeng Xu 1Yongyong Su 2Jinzheng Li 2Mang Zhu 3Maode Wang1
作者信息
- 1. First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710000, Shaanxi Province, China
- 2. Xianyang Central Hospital, Xianyang 712000, Shaanxi Province, China
- 3. Xi'an Central Hospital, Xi'an 710000, Shaanxi Province, China
- 折叠
摘要
Abstract
In the present study, cultured human SHG-44 glioma cells were subjected to a hypoxic environment simulated using the CoCl2 method. Flow cytometry showed increased reactive oxygen species production in these cells. Real-time reverse transcription-PCR showed significantly increased hypoxia-inducible factor-1α mRNA expression in cells exposed to the hypoxic condition. The antioxidant N-acetylcysteine significantly inhibited reactive oxygen species production and reduced hypoxia-inducible factor-1α mRNA expression in normoxic and hypoxic groups, especially in the latter group. These findings indicate that hypoxia induces reactive oxygen species production and hypoxia-inducible factor-1α mRNA expression in human SHG-44 glioma cells, and that the antioxidant N-acetylcysteine can inhibit these changes.关键词
neural regeneration/ basic research/ central nervous system/ reactive oxygen species/ hypoxia-inducible factor-1α/ hypoxia/ N-acetylcysteine/ glioma/ antioxidant/ photographs-containing paper/ neuroregeneraionKey words
neural regeneration/ basic research/ central nervous system/ reactive oxygen species/ hypoxia-inducible factor-1α/ hypoxia/ N-acetylcysteine/ glioma/ antioxidant/ photographs-containing paper/ neuroregeneraion引用本文复制引用
Haitao Jiang,Jiangtao Xie,Gaofeng Xu,Yongyong Su,Jinzheng Li,Mang Zhu,Maode Wang..Hypoxia regulates reactive oxygen species levels in SHG-44 glioma cells[J].中国神经再生研究（英文版）,2013,8(6):554-560,7.
