中国食品学报2012，Vol.12Issue(11)：160-169,10.
长双歧杆菌亚种JCM1217基因组分泌蛋白的分析
Genome-wide Analysis of the Secretory Proteins of Bifidobacterium Longum Subsp. JCM 1217
摘要
Abstract
Objective:Bifidobaeterium are important human intestinal probiotics, analysis of their secretory proteins will help us to correctly understand its physiological function and molecular mechanisms of interaction with their host. Methods:The amino acid sequence of Bifidobacterium longitm subsp. JCM 1217 genome proteins were obtained from Gen-Bank. Using SignalP v3.0, TMHMM 2.0, TatP, LipoPl.O, GPI, DOLOP software analyze the function of secretory proteins and the type and characteristic of these secretory proteins and their signal peptides. Results;A total of 58 secretion proteins through Sec pathway (including one through Tat pathway), 142 non-classical secretory proteins were screened. The secretory proteins from Sec pathway included secreted proteins recognized by Spase Ⅰ and Spase Ⅱ. Most of their signal peptide length are 25 and 28aa, respectively; The distribution and the average length of Spase Ⅱ secretory proteins were smaller than that of Spase I secretory proteins. The usage frequencies of alanine on the cleavage site left position -3, -2 and -1 of Bifidobacterium signal peptide and the mature protein were 61.11%, 19.4% and 83.33%, respectively, belonged to typically A-X-A signal peptide recognition sites structure. About to the functions of Bifidobacterium secretory protein, there were many hypothetical proteins in every type secretory proteins. Besides that, Spase Ⅰ type secretory proteins encoded a number of enzymes related to metabolism, while the hydrolyses were little; Spase Ⅱ type of secretory proteins are mainly related to ABC transporter proteins; the functions of non-classical secretory proteins were very complex, including enzymes with regard to metabolism and cell wall synthesis, DNA replication and gene expression related protein and other functional proteins. Conclusion;The secretory protein and their signal peptide from Bifidobacterium longwn subsp. JCM 1217 are similar to that from other Gram-positive bacteria. The functions of these secretory proteins can adapt to its host environment.关键词
长双歧杆菌/信号肽/分泌蛋白Key words
Bifidobacterium longum/ signal peptide/ secretory proteins引用本文复制引用
张秀红,王颖,段江燕..长双歧杆菌亚种JCM1217基因组分泌蛋白的分析[J].中国食品学报,2012,12(11):160-169,10.基金项目
山西省自然基金项目(2009011044-2) （2009011044-2）
