中国输血杂志2012，Vol.25Issue(12)：1283-1287,5.
不同吸附体系及因素水平对PCC中4类抗凝蛋白的影响研究
Effect of different adsorption system on the coagulation inhibitors in prothrombin complex concentrate
摘要
Abstract
Objective To study the effect of different adsorption systems for preparing prothrombin complex concen-trates (PCC) and their four variables (sodium citrate,NaCl,pH,and conductivity) on the yield and activity of proteins C (PC) ,S (PS) ,Z (PZ) ,and antithrombin (AT) during PCC production. Methods 9 adsorption conditions for purifying PCC were prepared according to the orthogonal table designed by three variables ( sodium citrate, NaCl, and pH) , and each in three levels. The gel was equilibrated by the corresponding adsorption condition,and then mixed with cryo-poor plasma to adsorb PCC. After the gel was washed, PCC was eluted. The PC, PS, and AT activity, PZ content, total volume and protein concentration of PCC were measured to obtain the yield and purity of the above four inhibitors, and then their yields and pu-rity in different adsorption conditions were compared. The effects of sodium citrate,NaCl and pH on the yield and purity of PC,PS,and PZ were analyzed by heuristics analysis method. In addition,the conductivity was determined and its impact on the yield and purity of the four coagulation inhibitors was analyzed. Results Under different adsorption conditions, the yield and purity of the four inhibitors were quite different. Both the yield arid the purity of AT were very low. Of the three varia-bles , sodium citrate had greater impact on PC yield and purity, as well as PS yield, and pH showed greater impact on PZ yield and purity,and PS purity. For three levels,the higher the sodium citrate content,the lower the yield and purity of PC, and the higher the yield of PS. When sodium citrate was 0. 015 mol/L,the yield of PZ was highest,but the purities of PS and PZ were the lowest. The lower pH, and the higher PZ yield. At pH7.0,the yield and purity of PC and PS,and the purity of PZ was the highest. When sodium chloride was 0. 075 mol/L,the PC yield and purity,and PS and PZ purity were the highest, but PS and PZ yields were the lowest. With the variation of conductivity, the PC yield and PZ purity showed greater fluctua-tion, furthermore the change tendency of PZ and S yield,PZ and S purity,and PC yield and purity,was similar. Conclusion Of all the adsorption systems,the yield and purity of AT are very low,and there is no association between the yield and purity of PC, PS, and PZ. Sodium chloride has less effect on the yield and purity. The effect of different levels of sodium cit-rate, NaCl,pH on their yield shows no uniform character, however their purity are the maximum at sodium citrate 0. 01mol/ L,NaCl 0. 075 mol / L,and pH 7. 0. There is no obvious linear relationship between the conductivity and the yield,as well as the purity of the four inhibitors.关键词
吸附体系/凝血酶原复合物/抗凝蛋白/收率/比活Key words
Adsorption system/Prothrombin complex concentrates/Coagulation inhibitors/Yield/Purity分类
医药卫生引用本文复制引用
曹海军,李长清,王宗奎,胡吉军,王玉梅,叶生亮,杜晞,张学俊,袁靖,林方昭..不同吸附体系及因素水平对PCC中4类抗凝蛋白的影响研究[J].中国输血杂志,2012,25(12):1283-1287,5.基金项目
2009年国家卫生行业科研专项(200902008-2) （200902008-2）
