中国实验动物学报2013，Vol.21Issue(1)：61-66,后插9-后插10,8.DOI:10.3969/j.issn.1005-4847.2013.01.012
A/H6N1亚型禽流感病毒致病性评估及与2009A/H1N1亚型流感病毒在哺乳动物体内的遗传兼容性
Evaluation of the pathogenicity of A/H6N1 avian influenza virus and its genetic compatibility with 2009 A/H1N1 pandemic influenza virus in mammalian models
摘要
Abstract
Objective A/H6N1 avian influenza virus has become an epidemic virus in poultry in China, posing a significant threat to public health. In order to evaluate the capacity of pathogenesis and reassortment of H6N1 virus in mammalians, we first evaluated the pathogenicity of H6N1 virus to mice, and then evaluated the compatibility between H6N1 virus and 2009 A/H1N1 pandemic viruse. Methods For the mouse infection experiment, the mice were inoculated intrana-sally with l01 EID50 - 106EID50 A/H6N1 AIV (A/Mallard/SanJiang/275/2007 , which was isolated from wild ducks) , and virological and histological data were collected by clinical sign observation, virus titration, and histopathology with HE staining. For the reassorment experiment in guinea pigs, the animals were inoculated with mixed A/H6N1 and A/H1N1 influenza viruses ( A/Changchun/01/2009 ) . The nasal washes of guinea pigs were collected every day, and reassortment viruses were obtained by plaque purification and identified by sequence analysis. Results In the mouse infection experiment, the mice were directly infected with H6N1 AIV virus, but were not lethal. The group of mice inoculated with 10 EID50 showed body weight loss, decreased activity, dorsal hair disordered, and tachypnea at day 15 after infection (DPI15). However, the clinical symptoms disappeared at DPI10. The virus titration results showed that the A/H6N1 virus mainly replicated in the lung, trachea and turbinate of mice, and the virus titers were up to 10 4,5/mL. Using HE staining, the pathological examination found that the lungs of the 10 EID50 group showed pathological changes, including alveolar wall thickening, infiltration of inflammatory cells, fibrin exudation and slight bleeding. In the reassorment experiment of guinea pigs, six reassortant virus strains were recovered with different gene segments derived from H6N1 and H1N1 viruses after three-time plaque purification. The results indicated that A/H6N1 and A/H1N1 influenza virus have high genetic compatibility in mammalian models. Conclusions A/H6N1 AIV can infect mammals directly without adaption and has a high genetic compatibility in guinea pigs with the H1N1 AIV. Therefore, attention should be paid on its significant threat to public health.关键词
A/H6N1亚型禽流感病毒/A/H1N1亚型流感病毒/致病性/兼容性Key words
H6N1/ avian influenza virus/ H1N1/ avian influenza virus/ Pathogenicity/ Compatibility/ Mouse/ Guinea pig分类
生物科学引用本文复制引用
程凯慧,王铁成,杨松涛,黄耕,赵永坤,高玉伟,华育平,夏咸柱,于志君,忻悦,张坤,丁洁,黄靖,岳秀芳,杨霞,乔军..A/H6N1亚型禽流感病毒致病性评估及与2009A/H1N1亚型流感病毒在哺乳动物体内的遗传兼容性[J].中国实验动物学报,2013,21(1):61-66,后插9-后插10,8.基金项目
国家重点基础研究发展计划 (2011CB505002),国家科技支撑计划 (2010BAD04B01). （2011CB505002）
