中国比较医学杂志2013，Vol.23Issue(1)：1-4,4.DOI:10.3969.j.issn.1671.7856.2013.001.001
猴免疫缺陷病毒衣壳蛋白p27在大肠杆菌中的表达及纯化
Expression and purification of SIV capsid p27 in E.coli
摘要
Abstract
Objective To obtain capsid p27 of SIV for detecting. Methods The p27 gene of SIV was synthesized and inserted into pMAL-p5x vector to construct pMAL-p5x-p27 recombinant plasmid. And then the recombinant plasmid was transformed into E. coli BL21 to express protein and the express product was purified by Amylose Resin maltose-binding protein affinity column. Results SDS-PAGE analysis indicated that pMAL-p5x-p27 can be expressed in E. coli BL21 and the protein molecular weight was about 70×103;The final p27 protein purification could reach 90% . Conclusion p27 of SIV was successfully expressed in E. coli,which lays a foudation for developing the methods of detecting SIV antibody.关键词
SIV/p27/表达/纯化Key words
SIV/ p27/ Prokaryotic expression/ Purification分类
医药卫生引用本文复制引用
李敏,曾林,马兰芝,白杰英,刘一,赵彦斌,王洪宝,丁璐静,武文卿,孙兆增..猴免疫缺陷病毒衣壳蛋白p27在大肠杆菌中的表达及纯化[J].中国比较医学杂志,2013,23(1):1-4,4.基金项目
十二五重大专项"高致病病原动物模型研究"(2012ZX10004502). （2012ZX10004502）
