中国比较医学杂志2013，Vol.23Issue(1)：23-26,4.DOI:10.3969.j.issn.1671.7856.2013.001.006
大鼠仙台病毒ELISA、间接免疫荧光和免疫印迹三种检测方法比较
A comparison of rat sendai virus detection methods of ELISA, IFA and WB
摘要
Abstract
Objective to compare the three methods of ELISA ,IFA and WB in the detection of Sendai virus in rat sera. Methods the SeV antigen proteins separated by SDS-PAGE were used in the WB method;20 sera from germ free rats,227 sera from SPF rats and 63 sera from clean rats were analyzed by ELISA ,IFA,those candidate positive samples were tested by WB. Results The 20 germ free rats were determined SeV negative by all of the 3 methods;all of the SPF rats were determined SeV negative by IFA,but 1. 32% of these SPF rats were determined SeV positive by ELISA,and 2/ 3 of these ELISA determined positive sera were confirmed by WB;The SeV positive rate in clean rats were 18. 12% by ELISA,11. 34% by IFA,and 15. 87% by WB. Conclusion the detection sensitivity gradient of the 3 methods from high to low is ELISA ,WB ,and IFA. And WB should be an alternative SeV detection method for those indeterminate samples by IFA and ELISA.关键词
仙台病毒/ELISA/IFA,Western blotKey words
Sendai virus/ ELISA/ IFA/ Western blot分类
医药卫生引用本文复制引用
向志光,佟巍,李雨函,刘先菊,张丽芳,王艳蓉,魏强..大鼠仙台病毒ELISA、间接免疫荧光和免疫印迹三种检测方法比较[J].中国比较医学杂志,2013,23(1):23-26,4.基金项目
中央级公益性科研院所基本科研业务费专项基金项目(DWS201213). （DWS201213）
