中国比较医学杂志2013，Vol.23Issue(2)：8-11,后插3-后插4,6.DOI:10.3969.j.issn.1671.7856.2013.002.003
小型猪肝硬化模型肝脏病理及生化指标的变化
Pathological and biochemical changes of the liver in mini-pig models of hepatic cirrhosis
摘要
Abstract
Objective To assess the pathological and biochemical changes of the liver during the establishment of mini-pig models of hepatic cirrhosis. Methods Fifty Bama mini-pigs of the same germline, male or female, aged 4 months, weighing 15 -20 kg, were included in this study. The mini-pigs were divided at random into an experimental group of 40 and a control group of 10 animals. Compound feed including carbon tetrachloride and phenobarbitone was employed to establish the mini-pig models in the experimental group, and normal feed was given to the control mini-pigs. Liver puncture biopsy was performed and the blood samples were taken from the ear vein at the end of 0th ,4th ,8th ,12th , 16th , 20th , 24th weeks for pathological and biochemical examinations, respectively. Results At the end of 24 th week, there were 70 stage 0 hepatic fibrosis in the normal group, while in the experiment group there were 82 slight hepatic fibrosis (stage S1 +S2) , 62 severe hepatic fibrosis (stage S3 + S4 ) , and 33 hepatic cirrhosis. From the normal to cirrhosis, the ALT, AST, T. BIL, CR and GLO were gradually elevated, ALB gradually decreased, A/G ratio inversed, and PT was gradually prolonged, with statistically significant differences between the different lesion groups ( P < 0. 01). Conclusions Compound feed including carbon tetrachloride and phenobarbitone can be used to successfully establish mini-pig models of hepatic cirrhosis, showing significant changes of liver pathology and biochemistry at different stages of the development of hepatic fibrosis.关键词
肝硬化/小型猪/病理学/生化指标Key words
Hepatic cirrhosis/ Hepatic fibrosis/ Mini-pig model/ Pathology, liver/ Biochemistry, liver分类
医药卫生引用本文复制引用
廖锦元,黄仲奎,黎宁钦,李佳梅..小型猪肝硬化模型肝脏病理及生化指标的变化[J].中国比较医学杂志,2013,23(2):8-11,后插3-后插4,6.基金项目
国家自然科学基金(编号:30760060). （编号:30760060）
