中国比较医学杂志2013，Vol.23Issue(2)：21-25,5.DOI:10.3969.j.issn.1671.7856.2013.002.006
Bio-Plex悬浮芯片系统检测两种实验兔白介素基因的差异表达
Detection of the differential expression of interleukin gene in two rabbit strains with liquid suspension array system
摘要
Abstract
Objective To measure the expression of IL-1β, IL-1R1 , IL-8 , IL-8RA and IL-15 genes in rabbit sacculus rotundus using the liquid suspension array system , and evaluate the application value of this method. Methods Twenty white hair black eyes ( WHBE) rabbits and twenty Japanes white ( JW) rabbits were used in this study. To simultaneously quantify the multiple genes mRNA expression in the rabbit sacculus rotundus by the Panomics QuantiGene Plex 2. 0 Assay of Affymetrix using the bDNA signal amplification and beads analysis techniques. Then , this established method was applied in the detection of rabbit interleukin gene. Results Content of IL-1β, IL-1R1 , IL-8, IL-8RA and IL-15 genes could be detected simultaneously. The IL-15 gene expression in WHBE rabbit was significantly higher than that in JW rabbit (P<0. 05) , and IL-1R1 gene expression in WHBE rabbits was significantly higher than that in JW rabbits ( P < 0. 01 ) . Also, the relative expression quantity of IL-8RA gene in WHBE rabbit was higher than that in JW rabbits (P < 0. 05 ) . Conclusion A liquid suspension array method for detecting rabbit interleukin gene has been established. The higher expressions of IL-15, IL-1R1 and IL-8RA genes in WHBE rabbits may be related to their unique immunological properties.关键词
液相悬浮芯片系统/定量/多重基因表达检测/bDNA/磁珠阵列Key words
Liquid suspension array system/ Quantitative/ Multi-gene expression detection/ bDNA/ Bead array分类
医药卫生引用本文复制引用
满曼,蔡月琴,徐剑钦,陈诚,黄宇,赵泓舒,杨钦钦,陈民利..Bio-Plex悬浮芯片系统检测两种实验兔白介素基因的差异表达[J].中国比较医学杂志,2013,23(2):21-25,5.基金项目
浙江省教育厅重大科技攻关项目(ZD2009004) （ZD2009004）
浙江省科技计划项目(2011C37093) （2011C37093）
受"浙江省卫生高层次创新人才培养工程项目"资助. （）
