摘要
Abstract
Objective Six-color flow cytometry(FCM)was used to detect minimal residual disease of B-lineage acute lymphoblastic leukemia.We aimed to investigate the relationship between clinical factors used by traditional risk-classification and MRD detected by six-color flow cytometry,and the relationship between the values of MRD delected at different times. Methods We included one group of six-color homotypic antibody combination IgG1-FITC/IgG1-PE/ IgG1-PE-Cy7/IgG1-APC/ IgG1 Percp/IgG1-APC-cy7 and two groups of six-color antibody combination based on im-munological classification as diagnosis CD20-FITC/ CD34-PE/CD10-PE-Cy7/CD22-APC/ CD45-Percp/ CD19-APC-cy7, CD7-FITC/ CD5-PE/ CD13-PE-Cy7/ CD33-APC/ CD45-Percp/ CD19-APC-cy 7 or CD38-FITC/CD34-PE/ CD 10-PE-Cy7/CD22-APC/CD45-Percp/CD19-APC-cy7,CD7 FITC/ CD15-PE/ CD34-PE-Cy7/CD3 3-APC/CD4 5-Per-cp/CD19-APC-cy7 , which were used to detect MRD of bone marrow specimens from 25 patients with leukemia-associated immune phenotype at diagnosis on 15 days, 33 days of induction chemotherapy and before high-dose MTX chemotherapy .The relationship between the value of MRD at each time point and clinical factors used for traditional risk classification , and the relationship of the value of MRD detection at three different times were analyzed. Results Among 25
B-ALL patients detected by six-color flow cytometry on 15 days of induction chemotherapy, 20 cases of 25 cases were detected MRD positive; the 25 percentile of MRD was 0.19%,the 50 percentile 0.67%,and the 75 percentile 4.21%.On 33 days of induction chemotherapy, 10 cases of 25 cases were detected MRD positive;the 25 percentile of MRD was 0.07%, the 50 percentile 0.10%,and the 75 percentile 0.26%.Before high-dose MTX chemotherapy 14 cases of 25 cases were detected MRD positive; the 25 percentile of MRD was 0.03%, the 50 percentile 0.04% , and the 75 percentile 0.09%. Conclusion In the induction chemotherapy phase, the positive rate of MRD at the end of induction chemotherapy, compared with MRD on day 15 of induction chemotherapy, is significantly decreased; the value of MRD before high-dose MTX chemotherapy, compared with 15 days and 33 days of induction chemotherapy, is gradually declined. According the factors used by traditional risk classification, the positive rate of MRD detection in each groupe is of no difference on 15 days of induction chemotherapy, at the end of induction chemotherapy and before high dose MTX chemotherapy.关键词
急性B淋巴细胞白血病/六色流式细胞术/微小残留病变Key words
acute B lineage lymphoblastic leukemia/six-color flow cytometry/minimal residual disease分类
医药卫生
