中国实用儿科杂志2013，Vol.28Issue(2)：107-110,4.
癫痫患儿外周血多药耐药基因动态表达及其意义研究
The dynamic expression and significance of multidrug resistance gene in the peripheral blood of children with epilepsy
摘要
Abstract
Objective To observe dynamic changes of the expression of multidrug resistance gene 1 (MDR1) in peripheral blood of children with epilepsy, and to explore the relationship between it and epileptic resistance and its value of predicting efficacy of antiepileptic drugs(AEDs).Methods Thirty children with refractory epilepsy, forty newly diagnosed epilepsy and thirty healthy children were collected. The expression of MDRlmRNA was analyzed by reverse transcription polymerase chain reaction(RT- PCR)semi-quantitatively before entering group, 6 months, 12 months and 18 months after treatment, respectively.The relationship of MDR1 level with epileptic frequency and therapeutic effect of AEDs was analyzed.Results The expression of MDR1 in refractory epilepsy group and newly diagnosed epilepsy group at every time point was all significantly higher than that in healthy control group (P < 0.01).The expression of MDR1 in refractory epilepsy group at every time point was significantly higher than that in newly diagnosed epilepsy (P< 0.01). There was a significant difference on the expression of the MDR1 in refractory epilepsy group at every time point (P< 0.01 ,P<0.05)and no significant difference in newly diagnosed epilepsy group . In refractory epilepsy group before entering group, 16 of 19 cases(84.2%)with over expression of MDR1 became invalid , 3 of 11 cases(27.3%)without expres- sion of MDR1 became invalid, and there was a significant difference between them (P< 0.01).In newly diagnosed epilepsy group before entering group, 9 of 13 cases (69.2%) with over expression of MDR1 became intractable epileptic patients and 2 of 27(7.5%) without expres-sion of MDR1 became intractable epileptic patients, and there was a significant difference between them (P< 0.01). MDR1 expression was more among patients with high frequent epilepsy than patients with low frequent epilepsy (P< 0.01 ).MDR1 expression was more in patients administered with four kinds of AEDs than those with two or three kinds of AEDs (P< 0.01).Conclusion The expression of MDR1 increases in peripheral blood of children with epilepsy, especially in refractory epileptic children. The increased expression of MDR1 may play an important role in clinical drug resistance. The expression of MDR1 may be a predictable marker in evaluating efficacy of AEDs.关键词
癫痫/抗癫痫药物/多药耐药基因/外周血/儿童Key words
epilepsy/ antiepileptic drugs/ multidrug resistance gene/ peripheral blood/ children分类
医药卫生引用本文复制引用
孔敏,蒋莉,洪思琪,胡君,叶园珍..癫痫患儿外周血多药耐药基因动态表达及其意义研究[J].中国实用儿科杂志,2013,28(2):107-110,4.基金项目
重庆市卫生局资助项目(2010-2-170) （2010-2-170）
