中国实用儿科杂志2013，Vol.28Issue(2)：114-117,4.
糖皮质激素受体基因多态性与汉族儿童哮喘关系研究
Association of the glucocorticoid receptor gene polymorphisms with childhood asthma in Han nationality
摘要
Abstract
Objective To investigate the genotypic frequency and the association of four SNPs(Bcl Ⅰ , N363S, ER22/ 23EK, Tth Ⅲ1) in the glucocorticoid receptor gene with childhood asthma in Han nationality. Methods The oral muco-sa swabs of 285 Han nationality children with asthma who had come to the outpatient of asthma from 2011 to 2012 were collected.The 4 single nucleotide polymorphisms (SNPs) were assessed by real-time polymerase chain reaction (PCR) using the TaqMan Allelic Discrimination (AD) assay.Besides, another 223 healthy subjects who came from Shanghai Jiao Tong University served as controls. Genotype and genotypic distribution between these two groups were analysed.Results There was no obvious difference in genotype and genotypic distribution of SNP loci of Bel Ⅰ , N363S, ER22/23EK or Tth Ⅲ 1 between asthmatic children and normal controls(P> 0.05). No aberrance was found in N363S or ER22/23EK of glucocorticoid receptor gene between asthma group and control group. Conclusion It seems that the 4 SNPs (Bel Ⅰ, N363S,ER22/23EK, Tth Ⅲ 1) in the glucocorticoid receptor gene do not play an important role in childhood asthma of Han ethnic. No N363S or ER22/23EK variants are found in Han people. It is also known that the frequency of N363S and ER22/23EK polymorphisms varies greatly between ethnic populations.关键词
哮喘/儿童/汉族/糖皮质激素受体/基因/单核苷酸多态性Key words
asthma/ childhood/ Han nationality/ glucocorticoid receptor/ gene/ single nucleotide polymorphisms分类
医药卫生引用本文复制引用
范飞,鲍一笑,刘全华,华丽..糖皮质激素受体基因多态性与汉族儿童哮喘关系研究[J].中国实用儿科杂志,2013,28(2):114-117,4.基金项目
上海市科委生物医学重大课题资助(10DZ1951000) （10DZ1951000）
