中国实用妇科与产科杂志2013，Vol.29Issue(1)：33-37,5.
耐药与敏感卵巢癌组织中microRNA表达差异谱检测及分析
Differentially microRNA expression detection between chemoresistance and sensitive ovarian cancer tissue and preliminary analysis
摘要
Abstract
Objective To test the microRNA (miRNA) expression of ovarian serous cystadenocarcinoma in both chemoresistance and sensitive tissue, and to explore the molecular mechanism of drug-resistant in ovarian serous cystadenocarcinoma involved the expression differences of miRNAs. Methods The samples each with 4 cases of tissue chemoresistance and sensitive ovarian serous cystadenocarcinoma were obtained from undergoing surgical resection of ovarian cancer. Total RNA and the small RNAs was extracted and isolated respectively. Hybridization was carried out on miRNA microarray chip. Real-time PCR was performed to confirm difference of miRNA expression level results obtained by microarray analysis between chemoresistance and sensitive ovarian serous cystadenocarcinoma within 4 cases each. Bioinformatic software was used to predict the possible target genes of each miRNAs which expressed differently. Results Three miRNAs related chemoresistance have been identified, 2 (has-miR-210, has-miR-1307) of them were up-regulated and 1 (has-miR-134) was down-regulated in ovarian serous cystadenocarcinoma tissues. The results obtained by miRNA microarrays of differential expression with has-miR-134, has-miR-210, has-miR-1307 were confirmed by real-time PCR. The target genes of has-miR-134 are possibly MRP1 /ABCC1, K-ras, Rho GDI2, after bioinformatics prediction, which of has-miR-210 are possibly HOXA9, FGFRL1, EFNA3,which of has-miR-1307 are possibly BCL-2, SCN5, CIC. Conclusion MiRNA microarray analysis has become a fast and efficient molecular biological technology for the study ofbiological information. Has-miR-134, has-miR-210 and has-miR-1307 may participate in the formation of drug-resistant in ovarian serous cystadenocarcinoma through the target genes predicted.关键词
卵巢肿瘤/耐药/microRNA微阵列芯片/实时监测聚合酶链反应Key words
ovarian tumor/ chemoresistance/ microRNA microarray chip/ real-time PCR分类
医药卫生引用本文复制引用
双婷,吴建磊,朱彦丽,史春雪,张萍,接智慧,常爽,王敏..耐药与敏感卵巢癌组织中microRNA表达差异谱检测及分析[J].中国实用妇科与产科杂志,2013,29(1):33-37,5.基金项目
国家自然科学基金(30973189) （30973189）
