中国实用妇科与产科杂志Issue(2)：123-127,5.
慢病毒介导的水通道蛋白8短发卡RNA抑制SiHa人子宫颈癌细胞的迁移研究
Aquaporin 8 short hairpin RNA mediated by lentivirus inhibits the migration of SiHa human cervical cancer cells
摘要
Abstract
Objective To probe the influence of AQP8 short hairpin RNA (shRNA) mediated by lentivirus on migration of SiHa human cervical cancer cells. Method To build stable transfection SiHa cell line of AQP8shRNA mediated by lentivirus, furtherly to detect inhibitory effect of AQP8 gene silence on migration of SiHa cells by transwell migration and invasion assays, wound healing assay and tumor cell growth, substrate adherence assays. Result (1) To successfully build stable transfection SiHa cell line of AQP8shRNA mediated by lentivirus; (2) Migration and invasion rate of AQP8shRNA-SiHa was obviously lower than control groups (P < 0.05) ; Wound healing speed of AQP8shRNA-SiHa was obviously lower than control groups (P<0. 05); Difference of growth and substrate adherence between AQP8shRNA-SiHa and control groups was not significant (P >0. 05). Conclusions Silence of AQP8 gene expression can inhibit migration of SiHa cells and potential invasive process.关键词
慢病毒/AQP8基因抑制/SiHa/肿瘤细胞迁移Key words
lentivirus / inhibition of AQP8 gene/ SiHa / tumor cell migration分类
医药卫生引用本文复制引用
史永华,尼加提·热合木,塔拉甫·托坎,拉莱·苏祖克..慢病毒介导的水通道蛋白8短发卡RNA抑制SiHa人子宫颈癌细胞的迁移研究[J].中国实用妇科与产科杂志,2013,(2):123-127,5.基金项目
国家自然科学基金地区基金(81060216) （81060216）
新疆医科大学科研创新基金(XJC201011) （XJC201011）
