中国兽医科学2013，Vol.43Issue(1)：42-46,5.
鸡毒支原体醛缩酶的原核表达及膜定位鉴定
Prokaryotic expression and membrane localization of aldolase of Mycoplasma gallisepticum
摘要
Abstract
On the basis of fructose-bisphosphate aldolase(fba) gene sequences of Mycoplasma gallisepticum available in GenBank, specific primers were designed and used for overlap PCR amplification from fba gene from M. Gallisepticum strain Rlow. The PCR product was cloned into the expression vector pET-28a( + ) and subsequently sequenced. The result showed that the open reading frame of fba gene was 873 bp in length, encoding 290 amino acids. The recombinant plasmid pET28a-fba was then transformed into Escherichia coli BL2KDE3) competent cells for expression via induction using IPTG. The rMGfba exhibited aldolase catalytic activity indicating that it was correctly expressed in vitro. Mouse antiserum was generated by immunization mouse with rMGfba. Western-blot assay showed that the antiserum could recognize a protein presented on the surface of M. Gallisepticum.关键词
鸡毒支原体/醛缩酶/原核表达/酶活性/膜定位Key words
Mycoplasma gallisepticum /aldolase/prokaryotic expression/enzymatic activity/membrane localization分类
农业科技引用本文复制引用
何随彬,谭磊,包世俊,郭小琴,仇旭升,宋翠萍,费荣梅,丁铲..鸡毒支原体醛缩酶的原核表达及膜定位鉴定[J].中国兽医科学,2013,43(1):42-46,5.基金项目
国家自然科学基金项目(30871883) （30871883）
公益性行业(农业)科研专项(201003012) （农业）
