中国兽医科学2013，Vol.43Issue(1)：47-52,6.
日本血吸虫重组多表位核酸疫苗的构建与免疫保护效果评估
Construction and immunoprotection evaluation of three multi-epitope DNA vaccines against Schistosoma japonicum
摘要
Abstract
The aim of the present study was to investigate the coordination effecting protective immunity in mice by connecting LHD-Sj23 with the epitopes of Sj28GST and SjGCP to form multi-epitope DNA vaccines. The epitopes of Sj28GST and SjGCP were analyzed and selected by bioinformatics method. The DNA fragments encoding LHD-Sj23, epitopes of Sj28GST and SjGCP were amplified by PCR,and then subcloned into the eukaryotic expression vector pCMV-Script to construct pCMV-BGCP-LHD-Sj23-BSj28, pCMV-LHD-Sj23-BSj28 and PCMV-BGCP-LHD-Sj23, respectively. All three plasmids were used to vaccinate Kunming mice by intramuscular injection. Mice were challenged by Schistosoma japonicum cercariae, and subsequently killed humanely for counting the number of the adult worm 42 days post infection. The three recombinant DNA vaccines induced worm reduction rates were 6. 30% ,14. 76% ,and 64. 95% respectively. The recombinant plasmid pCMV-BGCP-LHD-Sj23-BSj28 can induce the highest immunoprotection, indicating its potential to be an candidate vaccine against S. Japonicum. The results suggested that the multi-epitope vaccine may be a worthwhile way for vaccine development.关键词
日本血吸虫/表位/核酸疫苗Key words
Schistosoma japonicum/epitope/DNA vaccine分类
农业科技引用本文复制引用
章登吉,傅志强,石耀军,李浩,孙安国,彭运潮,林矫矫..日本血吸虫重组多表位核酸疫苗的构建与免疫保护效果评估[J].中国兽医科学,2013,43(1):47-52,6.基金项目
国家自然科学基金资助项目(31172315) （31172315）
公益性行业(农业)科研专项(200903036) （农业）
