中国体外循环杂志2013，Vol.11Issue(1)：41-44,4.
γ-分泌酶抑制剂对大鼠离体心脏缺血再灌注损伤的作用
Effects of γ-secretase inhibitor on isolated rat heart induced by ischemia reperfusion injury
摘要
Abstract
Objective DAPT is a specific inhibitor of Notch signaling pathway, our shirty investigated the effects of DAPT on isolated rat hearts induced by ischemia reperfusion injury (I/RI), and to explore the role of Notch signaling pathway in myocardial ischemia reperfusion injury (I/RI). Methods The hearts of SD rats were isolated, underwent Langendroff perfusion, and were divided into 4 groups: Control, I/RI, DAPT (1 ΜM) + I/RI, DAPT (1 ΜM). Myocardial injuries in the rat hearts were produced by ischemia for 50 min and followed by 60 min reperfusion. HR, LVDP, ± dp/dt max, CF and DP were recorded respectively before ischemia and 15, 30, 45 and 60 min after reperfusion; the myocardial infarct size (MI) was detected by TTC test; the special kits were used to detect the levels of lactic dehydrogenase (LDH) in the coronary flow (CF). Results In the I/R group, the systolic and diastolic functions significantly reduced (P < 0. 01), the MI and LDH in the CF increased significantly (P < 0. 01). Compared to the I/RI group, the above parameters in DAPT + IR group were worse (P <0. 01); compared to the Control group, the parameters in DAPT group had no significant difference (P > 0. 05). Conclusion DAPT can aggravate the myocardial I/RI, Notch signaling pathway may play a vital regulatory role in the myocardial reperfusion injury.关键词
γ-分泌酶抑制剂/离体大鼠心脏/缺血再灌注损伤/心肌保护Key words
DAPT/ Isolated rat heart/ Ischemia reperfusion injury/ Myocardial protection分类
医药卫生引用本文复制引用
杨阳,段维勋,王宁,梁振兴,姜鹏,周建,金振晓,易定华..γ-分泌酶抑制剂对大鼠离体心脏缺血再灌注损伤的作用[J].中国体外循环杂志,2013,11(1):41-44,4.基金项目
国家自然科学基金资助(81070198,81102687) 陕西省社会发展推动基金资助(2012JQ4001,2012SF2-21-1) （81070198,81102687）
