中国畜牧兽医2013，Vol.40Issue(1)：42-46,5.
猪嵴病毒RT-PCR检测方法的建立及应用
Establishment and Application of RT-PCR Detection of Pocrine Kobuvirus
摘要
Abstract
A detection method for porcine kobuvirus (PKV) with RT-PCR technique was established to detect the 3D gene of PKV from the intestinal tract of diarrhea piglets, with the detection limit about 2. 3 pg RNA. 50 samples of diarrhea piglets were detected by the RT-PCR method, the PKV positive rate of samples was 58%. The PCR detection method established in this study had the characteristics of sensitivity, high specificity and good repeatability, and could be used in molecular epidemi-ological investigation and rapid clinical diagnosis of PKV.关键词
猪嵴病毒/RT-PCR/同源性分析Key words
porcine kobuvirus/RT-PCR/homology analysis分类
农业科技引用本文复制引用
张文波,冷闯,段俊,邓舜洲..猪嵴病毒RT-PCR检测方法的建立及应用[J].中国畜牧兽医,2013,40(1):42-46,5.基金项目
国家自然科学基金项目(31160498/C1803). （31160498/C1803）
