中国畜牧兽医2013，Vol.40Issue(1)：57-61,5.
黄曲霉毒素B1残留ELISA试剂盒的研制及应用
Study and Application on the ELISA Kit for Detecting Aflatoxins B1
万宇平 1李勇 1王凤红 2韩京朋 1齐向武 1石丽丽1
作者信息
- 1. 北京勤邦生物技术有限公司,北京 102206
- 2. 北京挑战牧业科技股份有限公司,北京 100081
- 折叠
摘要
Abstract
This study was to aim to use ELISA method for rapid screening of the aflatoxins B1 (AFB1 ) residues. Coupled AFBi to bull serum albumin(BSA) and ovalbumin(OVA) to obtain AFB1-BSA and AFB1-OVA respectively. Specific antibody against AFB1 was prepared with AFB1-BSA and ELISA kit, and had been successfully developed for detecting AFBi residues. The IC50 of the kit was 128. 8 ng/L,linear detection range was 50～1800 ng/L with the lowest detection limit less than 100 ng/kg. The recovery of AFB1 in edible oil,peanut and cereal was more than 71. 3%. Coefficient of variation for repeatability tests were less than 14. 2%. Therefore,the kit was simple,fast,sensitive,and would be play an important role in AFB1 residues detection in the plant food.关键词
黄曲霉毒素B1/酶联免疫吸附试验/试剂盒Key words
aflatoxins B1/enzyme-linked immunosorbent assay/kit分类
医药卫生引用本文复制引用
万宇平,李勇,王凤红,韩京朋,齐向武,石丽丽..黄曲霉毒素B1残留ELISA试剂盒的研制及应用[J].中国畜牧兽医,2013,40(1):57-61,5.
