中国畜牧兽医2013，Vol.40Issue(1)：81-84,4.
青海高原半细毛羊白细胞介素-8基因克隆及序列分析
Cloning and Sequence Analysis of IL-8 Gene in Qinghai Semifine-wool Sheep
柳存1
作者信息
- 1. 青海省海晏县青海湖乡兽医站,青海海晏 812200
- 折叠
摘要
Abstract
This experiment was conducted to study molecular evolution, cloning and sequence analysis of Qinghai Semifine-wool sheep interleukin-8 (IL-8) gene. A pair of specific primers was designed based on GenBank. The IL-8 gene was amplified by RT-PCR and cloned into pMD18-T vector. The positive recombinant plasmids which was transformed to E. Coli DH5α was identified by PCR and restriction enzyme digestion and sequenced analysis. The results showed that sheep peripheral blood lymphocytes was amplified bands consistent with the expected size (370 bp) , the homology analysis showed that IL-8 gene was more conservative, which homology was 96. 1 % to 99. 0%.关键词
IL-8基因/克隆/序列分析/半细毛羊Key words
IL-8 gene/cloning/sequence analysis/Semifine-wool sheep分类
生物科学引用本文复制引用
柳存..青海高原半细毛羊白细胞介素-8基因克隆及序列分析[J].中国畜牧兽医,2013,40(1):81-84,4.
