中国畜牧兽医2013，Vol.40Issue(1)：145-148,4.
4个品种鸡Toll样受体4基因第2内含子的多态性分析
Polymorphisms Analysis of Toll-like Receptor 4 Gene Intron 2 in Four Different Chicken Breeds
摘要
Abstract
The genetic distribution of the polymorphisms of intron 2 of TLR4 gene in four different chicken breeds including Xianju, Baier, Qingyuan, Anka chicken were tested by PCR-SSCP and DNA sequencing in this study. The results indicated that a G1894C mutation existed in four different chicken breeds. There were three genotypes of AA, AB and BB in all the breeds. The results showed that inspected locus was high diversity,the average polymorphism information content (PIC) were Xianju chicken 0. 570, Baier chicken 0. 597, Qingyuan chicken 0. 583, and Anka chicken 0. 588, respectively, and were accorded with the law of Hardy-Weinberg equilibrium. The genotypes of G1894C polymorphism locus was no significant differences between the positive and negative groups after chickens infected with Salmonella pullorum (P>0. 05). Genotype distribution of G1894C polymorphism locus was highly significant difference between Xianju and Baier chicken (P<0. 01), which would provide scientific basis and technological supporting for resistance-related gene analysis in future.关键词
鸡/TLR4基因/单核苷酸多态性Key words
chicken/TLR4 gene/single nucleotide polymorphisms分类
农业科技引用本文复制引用
陶志云,徐文娟,宋卫涛,宋迟,胡艳,单艳菊,陈宽维,李慧芳..4个品种鸡Toll样受体4基因第2内含子的多态性分析[J].中国畜牧兽医,2013,40(1):145-148,4.基金项目
现代农业肉鸡产业技术体系岗位专家(CARS-42-GOG) （CARS-42-GOG）
国家高技术研究发展计划(863)项目(2011AA100301) （863）
江苏省自然科学基金(BK2010296) （BK2010296）
国家自然科学基金青年科学基金项目(31101795). （31101795）
