中国畜牧兽医2013，Vol.40Issue(1)：153-157,5.
牦牛产志贺毒素大肠杆菌主要黏附因子相关基因的分子流行病学调查
Molecular Epidemiological Investigation of Genes Related with the Major Adhesion Factors in Shiga Toxin-producing Escherichia coli from Yak
摘要
Abstract
The assay was aimed to investigate the prevalence of the major adhension factors in Shiga toxin-producing Escherichia coli from Yak. By using the method of PCR to detect the adhension factors of eae,saa,iha genes in the 70 STEC isolates from the healthy Yak in Sichuan Ganzi, Aba and other places,some of the adhension genes were cloned and sequenced. The results showed that the carried rates of saa and iha genes were 71. 42% (50/70) and 78. 57% (55/70) respectively in 70 STEC strains from Yak, none of the STEC isolates carried the eae gene(0/70), the homologies of saa and iha genes were 100% and 93% to 99% respectively with the reference sequences in GenBank. STEC isolates from the healthy Yak didn't have eae gene which was encoded by LEE virulence island, the carried rates of other adhension virulence genes saa, iha were higher.关键词
牦牛/产志贺毒素的大肠杆菌/黏附基因Key words
Yak/Shiga toxin-producing Escherichia coli/adhension gene分类
农业科技引用本文复制引用
宋定州,于学辉,汤承,李键,冉丹丹..牦牛产志贺毒素大肠杆菌主要黏附因子相关基因的分子流行病学调查[J].中国畜牧兽医,2013,40(1):153-157,5.基金项目
中央高校基本科研业务费科研平台建设项目(11NPT03) （11NPT03）
国家公益性行业(农业)科研专项经费(201203009). （农业）
