中国烟草学报2012，Vol.18Issue(6)：53-59,7.DOI:10.3969/j.issn.1004-5708.2012.06.011
烤烟轮作与连作土壤细菌群落多样性比较
Comparison of bacteria diversity between tobacco plantation soils of rotational cropping and continuous cropping
摘要
Abstract
Total DNA of microbe in rotational cropping and continuous cropping soil were extracted and amplified by PCR with universally bacteria-specific rDNA primers. Clone libraries were set up to evaluate responses of soil bacteria community to different tobacco cropping systems (rotational and continuous cropping). PCR-RFLP analysis was carried out with restriction endonuclease Hae III and Afa I. 147 and 177 types of restriction endonuclease were obtained from these samples. Statistical analysis using diversity index measurement showed both Shannon-wiener index and species richness of rotational cropping soil were higher than that in continuous cropping soil. Bacterial phylogenetic trees of the samples revealed that Acidobacteria was dominant group in continuous cropping soil library while acidobacteria,γ-pro-teobacterium, α-proteobacterium were in rotational cropping soil library. It indicated that rotational farming system could improve bacterial diversity of soil while continuous cropping could simplify bacterial community structure. This phenomenon might cause functional disorder in bacterial community and destruction of ecological balance in rhizosphere soil. It might be one of the factors resulted in continuous cropping obstacle.关键词
连作/轮作/克隆文库/RFLP分析Key words
rotational cropping, continuous cropping, clone library, RFLP analysis分类
农业科技引用本文复制引用
段玉琪,晋艳,陈泽斌,夏振远,杨宇虹,徐照丽..烤烟轮作与连作土壤细菌群落多样性比较[J].中国烟草学报,2012,18(6):53-59,7.基金项目
云南省烟草专卖局(公司)科技项目(07A05,08A02,2010YN05) （公司）
