中国药房2012，Vol.23Issue(45)：4233-4235,3.DOI:10.6039/j.issn.1001-0408.2012.45.04
紫杉醇对肺癌细胞株A549 Wnt/β-catenin信号通路相关基因和蛋白表达的影响
Effects of Paclitaxel on Gene and Protein Expression of Lung Cancer Cell Line A549 Wnt/β-catenin Signaling Pathway
王永富 1秦治明1
作者信息
- 1. 重庆医科大学附属第一医院心胸外科,重庆400016
- 折叠
摘要
Abstract
OBJECTIVE: To explore the effects of paclitaxel on gene and protein expression of lung cancer cell line molecular A549 Wnt/β-catenin signaling pathway. METHODS: β-catenin protein of A549 cell expression of 1 nmol·L-1 paclitaxel group and control group were determined by immunohistochemical method. RT-PCR method was used to detect dkk-3 (cancer suppressor gene) and c-myc (primary cancer gene) mRNA expression level of A549 cell in 0.1, 1 and 10 nmol·L-1 paclitaxel groups and control group for 48 h. RESULTS: Compared with control group, the expressions of β-catenin protein and c-myc mRNA were all down-regulated in 0.1, 1, 10 nmol·L-1 paclitaxel groups, while dkk-3 mRNA expression was up-regulated significantly (all P< 0.05) in concentration-dependant manner. CONCLUSION: Paclitaxel could block Wnt signaling pathway and reduce the expression of c-myc by down-regulating protein expression of β-catenin and inducing dkk-3 mRNA expression of lung cancer cell line A549.关键词
紫杉醇/Wnt信号通路/A549细胞Key words
Paclitaxel/ Wnt signaling pathway/ A549 cell分类
医药卫生引用本文复制引用
王永富,秦治明..紫杉醇对肺癌细胞株A549 Wnt/β-catenin信号通路相关基因和蛋白表达的影响[J].中国药房,2012,23(45):4233-4235,3.
