中国药房2012，Vol.23Issue(47)：4422-4424,3.DOI:10.6039/j.issn.1001-0408.2012.47.03
鬼臼毒素衍生物YB-1EPN对人口腔鳞状上皮癌细胞系增殖的抑制作用研究
Inhibitory Effect of Podophyllotoxin Derivative (YB-1EPN)Against the Growth of Human Oral Squamous Epithelium Caner Cell
摘要
Abstract
OBJECTIVE: To study the inhibitory effect of podophyllotoxin derivative (YB-1EPN) against the growth of human oral squamous epithelium cancer cell (KB). METHODS: MTT assay was used to detect cytotoxic effect of KB cell; Fluorescent staining method was used to observe the changes of cell morphology; expression of caspase-3 mRNA were measured by RT-PCR assay, and the effect of drugs on microtubules was observed by laser scanning microscope. RESULTS: YB-1EPN(4 μmol·L-1)was demonstrated to exert antiproliferative activity in dose and time dependent manner. Typical apoptotic morphological changes were observed by fluorescence microscope; RT-PCR assay showed that YB-1EPN(1,2,4 μmol·L-1)up-regulated mRNA expression of Caspase-3 dramatically, and the structure of microtubulin was destroyed by laser confocal microscope. CONCLUSION: YB-1EPN show obvious anticancer activity by inducing apoptosis in the dose-dependent manner, which are accompanied by up-regulating Caspase-3 and inhibiting the microtubule polymerization.关键词
鬼臼毒素衍生物/微管蛋白/半胱天冬氨酸蛋白酶-3/增殖/抑制Key words
Podophyllotoxin derivative/ Microtubulin/ Caspase-3/ Growth/ Inhibitory effect分类
医药卫生引用本文复制引用
毕文超,刘世军,孙克明,刘宪勇,张敏..鬼臼毒素衍生物YB-1EPN对人口腔鳞状上皮癌细胞系增殖的抑制作用研究[J].中国药房,2012,23(47):4422-4424,3.基金项目
国家自然科学基金资助项目(30873363) （30873363）
