中国药房2012，Vol.23Issue(47)：4430-4432,3.DOI:10.6039/j.issn.1001-0408.2012.47.06
山柰酚静脉注射后在大鼠体内的药动学研究
Pharmacokinetic Study of Kaempferol in Rats with Intravenous Administration
摘要
Abstract
OBJECTIVE: To investigate the pharmacokinetic characteristics of kaempferol in rats with intravenous administration. METHODS:60 rats were randomly divided into 10 time groups with 6 rats in each group. They were given medicine 5 mg·kg-1 intravenously(0.1 mL·kg-1). The blood samples 0.5 mL were collected from the rats at 1, 3, 5, 10, 20, 40, 80, 120, 180 and 240 min in each time group(n=6). The plasma level of kaempferol was determined by HPLC. The determination was performed on Phenomenex C18(100 mm×4.6 mm, 6 μm) column and Dikma Easy II Guard C18 Kit (8 mm×4 mm) pre-column with mobile phase consisted of acetonitrile-water (1:2, V/V) at the flow rate of 0.5 mL·min-1. The detection wavelength was set at 370 nm and column temperature was 35 ℃. The injection volume was 80 μL. Pharmacokinetic parameters were calculated with 3p97 software. RESULTS: The plasma concentration-time curves of kaempferol were presented by two-compartment model in rats. The main pharmacokinetic parameters were as follows: t1/2 α = (0.95 ± 0.35) min; t1/2=(5.68 ± 0.94) min; AUC0→t=( 155.10 ± 7.43) μg·mm·mL-1; AUC0→∞= (199.84± 14.07) μg·min·mL-1;CL=(45.90± 1.90) mL·kg-1·min-1. CONCLUSION: Original kaempferol is eliminated quickly in rats, and the concentration of drug decrease immediately after administration. More than 95 % of the original kaempferol is eliminated in 30 min.关键词
山柰酚/大鼠/高效液相色谱法/药动学Key words
Kaempferol/ Rat/ HPLC/ Pharmacokinetics分类
医药卫生引用本文复制引用
孔令希,李秀英,何海霞,周远大,杨辉,李娟..山柰酚静脉注射后在大鼠体内的药动学研究[J].中国药房,2012,23(47):4430-4432,3.基金项目
重庆市卫生局中医药科技项目(2009-1-6) （2009-1-6）
