中国药房2013，Vol.24Issue(1)：27-29,3.DOI:10.6039/j.issn.1001-0408.2013.01.09
氯胺酮对幼龄小鼠自主活动的影响和致遗忘作用及其与GABAA、NMDA受体的关系
Effects on Spontaneous Motor Activity and Amnestic Effect of Ketamine and Its Relationships with GABAA and NMDA Receptors in Young Mice
摘要
Abstract
OBJECTIVE: To investigate the effects of ketamine (Ket) on spontaneous motor activity and amnestic effect of Ket and its relationships with γ-aminobutyric acid (GABAA) and N-methyl-D-aspartic acid (NMDA) receptor in young mice. METHODS: Young mice were divided into 3 groups for spontaneous motor activity test, step-through test and step-down test. Every group was subdivided into 8 subgroups (n= 10), i.e. group A (NS+aCSF), group B (Ket+aCSF), group C and D [NS+GABAA receptor specific blocker bicuculline (Bic) 0.10 and 0.20 mg/kg], group E and F (Ket+Bic 0.10 and 0.20 mg/kg), group G (NS+ NMDA 60 ng), group H (Ket+NMDA 60 ng). Intraperitoneal injection of Ket 100 mg/kg or NS were given 20 min before test, and intralateroventricular injection of aCSF, Bic or NMDA were given 5 min before test. The spontaneous motor activities of mice were evaluated by spontaneous motor activity times (SMATs) within 5 min, and the amnestic effect of mice in 48 h after training was evaluated by error times (ETs), step-through latency (STL) and step-down latency (SDL). RESULTS: Compared with group A, SMATs increased significantly in group B, E, F, and H (P<0.05 or P<0.01). Compared with group B, SMATs decreased significantly in group C and D (P<0.05 or P<0.01). Compared with group A at 24 h after training, ETs increased in group B, E and F while STL and SDL decreased significantly in step-through test and step-down test; compared with group B, ETs decreased in group C, D, G and H while STL and SDL increased significantly (P<0.05 or P<0.01). Above index had no significant difference among those groups at 48 h after training. CONCLUSIONS: Ket can increase the SMATs and have amnestic effect in young mice. NMDA receptor but not GABAA receptor may be an important target for the amnestic effect in young mice induced by Ket.关键词
氯胺酮/幼龄小鼠/荷包牡丹碱/γ-氨基丁酸A/N-甲基-D-天冬氨酸/自主活动/遗忘作用Key words
Ketamine/ Young mice/ Bicuculline/ γ-aminobutyric acid A/ N-methyl-D-aspartic acid/ Spontaneous motor activity/ Amnestic effect分类
医药卫生引用本文复制引用
高艳,张洋,赵佳佳,陈雪,高震,李伟,傅焓,张科学,戴体俊,王红军..氯胺酮对幼龄小鼠自主活动的影响和致遗忘作用及其与GABAA、NMDA受体的关系[J].中国药房,2013,24(1):27-29,3.基金项目
国家自然科学基金资助项目(No.30872432,30471657,39970715) （No.30872432,30471657,39970715）
江苏省自然科学基金资助项目(No.BK200143) （No.BK200143）
