中国药房2013，Vol.24Issue(1)：30-32,3.DOI:10.6039/j.issn.1001-0408.2013.01.10
一步阳离子柱层析高效纯化溶菌酶及其溶菌活性
Efficient Purification of Lysozyme by One-step Cation Column Chromatography and Its Bacteriolytic Activity Assay
摘要
Abstract
OBJECTIVE: To study on how to purify lysozyme highly and efficiently from egg white by ion exchange chromatography. METHODS: The egg white was diluted and then precipitated by isoelectric precipitation to remove ovalbumin and ovomu-cin, etc. The supernant flowed into ethyloic-agarose gel for ion exchange chromatography. Samples of elution peak was collected and freeze-dried. SDS-PAGE and bacteriolytic turbidimetry were used to identify the purity and activity of the products, respectively. RESULTS: After isoelectric precipitation and one-step column chromatography, the purity of the products was over 95% ,the specific activity was 17 600 U/mg,and the yield achieved 4.2 mg (lysozyme) per ml (egg white). CONCLUSION:One-step cation column chromatography can be applied to acquire high-quality lysozyme efficiently from egg white.关键词
一步/阳离子柱层析/蛋清/纯化/溶菌酶/溶菌活性Key words
One-step/ Cation column chromatography/ Egg white/ Purify/ Lysozyme/ Bacteriolytic activity分类
医药卫生引用本文复制引用
赵林,蔡金艳,周林,李黄金,周联..一步阳离子柱层析高效纯化溶菌酶及其溶菌活性[J].中国药房,2013,24(1):30-32,3.基金项目
广东省科技计划资助项目(No.2011B080701004) （No.2011B080701004）
