中国药房2013，Vol.24Issue(2)：130-132,3.DOI:10.6039/j.issn.1001-0408.2013.02.14
超滤法配合LC-MS/MS法监测人血浆中游离型阿德福韦的浓度
Monitoring of Free Adefovir in Human Plasma by Ultrafiltration Coupled with LC-MS/MS Method
谢展雄 1陈桂红 2彭巧华 1吴铁松 1温中明3
作者信息
- 1. 深圳龙华新区观澜人民医院,广东深圳518110
- 2. 深圳市宝安区西乡人民医院,广东深圳518102
- 3. 深圳光明新区人民医院,广东深圳518106
- 折叠
摘要
Abstract
OBJECTIVE: To establish a fast, accurate, sensitive and specific, method for the determination of free adefovir in human plasma. METHODS: Using PMPA as the internal standard, ultrafiltration was adopted for sample process and coupled with LC-MS/MS. The detection condition was as followe: positive ion mode detection, MRM mode scan and detection channels m/z 274.2→226.1 and m/z 288.4→176.2 for adefovir and PMPA, respectively. RESULTS: The retention time of adefovir and PMPA were 3.78 min and 3.54 min, respectively. The linear ranges of adefovir were 0.25-50.00 ng/ml, and limit of quantification was 0.25 ng/ml. The average recoveries were 92.2%-93.2%, and RSD of intra-day and inter-day were less than 10%. RSD of stability test were all less than 10%. CONCLUSIONS: The analysis time of the method is short and the method has high specificity, accuracy. It can be used for plasma concentration monitoring of adefovir.关键词
阿德福韦/高效液相色谱-串联质谱法/超滤法/游离型/血药浓度Key words
Adefovir/ LC-MS/MS/ Ultrafiltration/ Free/ Plasma concentration分类
医药卫生引用本文复制引用
谢展雄,陈桂红,彭巧华,吴铁松,温中明..超滤法配合LC-MS/MS法监测人血浆中游离型阿德福韦的浓度[J].中国药房,2013,24(2):130-132,3.
