中国药房2013，Vol.24Issue(3)：216-219,4.DOI:10.6039/j.issn.1001-0408.2013.03.08
欧前胡素在大鼠体内的药动学与组织分布研究
Study on Pharmacokinetics and Tissue Distribution of Imperatorin in Rats
张笑恺 1曹蔚 1谢艳华 1段林瑞 1王四旺1
作者信息
- 1. 第四军医大学药学系药物研究所,西安710032
- 折叠
摘要
Abstract
OBJECTIVE: To establish the method for the determination of imperatorin in biological samples, and to study the pharmacokinetics and tissue distribution of imperatorin in rats. METHODS: The analytes were prepared by a liquid-liquid extraction method. Rats were given imperatorin, blood concentration of tamoxifen was determined by HPLC-UV. The separation was performed on Hypersil BDS C18(250 mm×4.6 mm, 5 μm) using acetonitrile-water (60:40, V/V)as mobile phase at flow rate of 1.0 ml/ min. The column temperature and ultraviolet detection were performed at 25 ℃ and 300 nm. Isoimpera好torin was used as the internal stand.The data were calculated by DAS2.0 software. The tissue distribution of imperatorin were studied. RESULTS: Good linear calibration curves for the plasma and tissues were obtained (r≥0.998 4), and the recovery was greater than 87.69% for each sample. The intra-day and inter-day RSDs were 0.58%-6.15% and 0.53%-8.00%, and RSDs of stability were 0.56%-6.99%. The concentration-time curve of imperatorin in rat plasma could be fitted to two-compartment model (W=1) ,tmax=2.5 h, Cmax=( 1.45 ± 0.49) mg/ L, AUC0-∞ = (7.03 ± 2.27) mg/(h·L) ;and imperatorin was widespread in tissues. CONCLUSION: The method is simple, rapid, sensitive and accurate, and is suitable for pharmacokinetics and tissue distribution study of imperatorin in plasma and tissues of rats.关键词
欧前胡素/白芷/高效液相色谱法/药动学/组织分布Key words
Imperatorin/ Angelica dahurica/ HPLC/ Pharmacokinetics/ Tissue distribution分类
医药卫生引用本文复制引用
张笑恺,曹蔚,谢艳华,段林瑞,王四旺..欧前胡素在大鼠体内的药动学与组织分布研究[J].中国药房,2013,24(3):216-219,4.
