中国药房2013，Vol.24Issue(3)：254-256,3.DOI:10.6039/j.issn.1001-0408.2013.03.22
60Co-γ射线辐照前后大黄饮片中5种成分的含量变化研究
Research of Content Change for 5 Components in Rhei Radix et Rhizoma Decoction Pieces before and after Irradiation by 60Co-γ Ray
李月梅 1刘卫红 2张娜娜 3张振巍1
作者信息
- 1. 解放军第155中心医院,河南开封475003
- 2. 河南中医学院第一附属医院,郑州 450000
- 3. 开封市中医院,河南开封730030
- 折叠
摘要
Abstract
OBJECTIVE: To study the content change for 5 active components in Rhei Radix et Rhizoma decoction pieces before and after the irradiation by 60CO-γ ray. METHODS: Rhei Radix et Rhizoma decoction pieces were collected after the irradiation by normal dose of 60CO-γ ray. The contents of 5 main active components were determined by HPLC method (aloe-emodin, em-odin, rhein, chrysophanol, physcion). The determination was performed on SPHERIGEL ODS C18(150 mm×4.6 mm,5 μm) with mobile phase consisted of methanol-0.1 % phosphoric acid (80:20, V/V). The detection wavelength was set at 254 nm. RESULTS: The content of emodin in Rhei Radix et Rhizoma decoction pieces was the highest; followed by physcion and chrysophanol; the contents of aloe-emodin was the lowest. After irradiation, the contents of each component were lower than before, especially physcion; while less that of aloe-emodin. After half year, the main active components of un-irradiated Rhei Radix et Rhizoma decoction pieces decreased to different extents; the contents of main components in Rhei Radix et Rhizoma decoction pieces decreased to some extents after irradiation by 60CO-γ ray, and stability of the components increased. CONCLUSION: The effects of ray on chemical components should be considered during medicinal material or preparation sterilization. The index of preparations should be re-detected and analyzed quantitatively after sterilization to guarantee the contents of active components within national standard.关键词
大黄/60Co-γ射线辐照/有效成分/含量变化/高效液相色谱法Key words
Rhei Radix et Rhizoma/60CO-γ ray irradiation/ Active component/ Content change/ HPLC分类
医药卫生引用本文复制引用
李月梅,刘卫红,张娜娜,张振巍..60Co-γ射线辐照前后大黄饮片中5种成分的含量变化研究[J].中国药房,2013,24(3):254-256,3.
