中国药房2013，Vol.24Issue(5)：407-409,3.DOI:10.6039/j.issn.1001-0408.2013.05.09
不同浓度二甲基亚砜对白色念珠菌的抑菌作用研究
Inhibitory Activity of Different Concentrations of Dimethyl Sulfoxide against Candida albicans
摘要
Abstract
OBJECTIVE: To investigate the inhibitory activity of different concentrations of dimethyl sulfoxide (DMSO) on Candida albicans. METHODS: C. albicans was separately cultured with dilution method in medium containing 0.14, 0.42, 0.70, 0.99, 1.27 and 1.55 mol/L DMSO for 24 h, using DMSO as positive control and liquid sabouraud medium as negative control. The inhibitory rates of C. albicans growth in different concentrations of DMSO and germ tube germination were calculated. RESULTS: The growth of C. albicans was active when the concentration of DMSO was 0.14 mol/L. There was no significant difference in inhibitory rates of C. albicans growth and germ tube germination between 0.14 mol/L group and negative control group (P>0.05); when the concentration of DMSO was higher than 0.42 mol/L, MIG90 of C. albicans was 0.70 mol/L during 2-4 h and MIG90 of germ tube germination was 0.70 mol/L; when the concentration of DMSO was up to 1.55 mol/L, there is no bacterial survival (inhibitory rate of bacteria growth reached 100%). When the concentration of DMSO was higher than 0.42 mol/L, there was significant difference in inhibitory rates of C. albicans growth and germ tube germination among DMSO medium groups and negative control group (P<0.05). CONCLUSIONS: Higher than 0.42 mol/L, DMSO has a strongly inhibitory activity on the growth and germ tube germination of C. albicans. With the concentration and time increasing, DMSO shows more apparent inhibitory effect.关键词
二甲基亚砜/白色念珠菌/抑菌作用/芽管萌发Key words
DMSO/ Candida albicans/ Inhibitory activity/ Germ tube germination分类
医药卫生引用本文复制引用
崔光富,刘瑞琪,郭巧梅,崔晶晶,贺晓丹,郭庆合,贺志安..不同浓度二甲基亚砜对白色念珠菌的抑菌作用研究[J].中国药房,2013,24(5):407-409,3.基金项目
河南省教育厅课题项目资助(No.2009B310004) （No.2009B310004）
新乡医学院2010年大学生科研创新项目资助 （）
新乡医学院2011年大学生科研课题资助项目(No.DXSKYKT2011-017) （No.DXSKYKT2011-017）
