中国药房2013，Vol.24Issue(5)：431-434,4.DOI:10.6039/j.issn.1001-0408.2013.05.17
星点设计-效应面法优化α-细辛脑脂质体喷鼻剂的制备工艺
Optimization of Preparation Technology of α-Asarone Liposome Nasal Spray by Central Composite Design-response Surface Methodology
摘要
Abstract
OBJECTIVE: To optimize the preparation technology of a-Asarone liposome nasal spray by central composite design-response surface methodology. METHODS: a-Asarone liposome nasal spray was prepared by ethanol injection method. A central composite design-response surface methodology was used to optimize the preparation technology. The formulation and technology were optimized by single factor test using the concentration of phospholipid, weight ratio of phospholipid to cholesterol and weight ratio of phospholipid to drug as independent variables, entrapment efficiency as dependent variable. The optimal technology was validated and the morphology of liposome was observed under electron microscope. RESULTS: The adjusted R2 (R2=0.907 4) value was high, indicating that the model was well adapted to the responses. The optimum technology was that phospholipid was 4.5% , weight ratio of phospholipid to cholesterol was 7.5, weight ratio of phospholipid to drug was 45 and entrapment efficiency was 68.1%. The deviation of results of optimal technology and predicted value of binominal fitted equation was 1.73%. Prepared liposome was complete in appearance with clear bimolecular film structure, all belonged to unilamelar liposome. CONCLUSIONS: Central composite design-response surface methodology is successfully used to optimize the preparation technology of a-Asarone liposome nasal spray. The optimized technology is stable and feasible.关键词
α-细辛脑/脂质体/喷鼻剂/制备/星点设计/效应面法Key words
α-Asarone/ Liposome/ Nasal spray/ Preparation/ Central composite design/ Response surface methodology分类
医药卫生引用本文复制引用
陈颖,黄海潮,朱庆玲,祝红,伍善广..星点设计-效应面法优化α-细辛脑脂质体喷鼻剂的制备工艺[J].中国药房,2013,24(5):431-434,4.基金项目
广东食品药品职业学院自然科学项目资助(No.2010YZ006) （No.2010YZ006）
