中国药房2013，Vol.24Issue(5)：446-447,2.DOI:10.6039/j.issn.1001-0408.2013.05.22
RP-HPLC法同时测定复方曲安奈德乳膏中醋酸曲安奈德和盐酸苯海拉明的含量
Content Determination of Triamcinolone Acetonide and Diphenhydramine Hydrochloride in Compound Triamcinolone Acetonide Cream by RP-HPLC
陈晓峰 1张咏梅 1汪明志2
作者信息
- 1. 张家港市中医医院,江苏张家港215600
- 2. 南京中医药大学药学院,南京210046
- 折叠
摘要
Abstract
OBJECTIVE: To establish a method for the content determination of triamcinolone acetonide and diphenhydramine hydrochloride in Compound triamcinolone acetonide cream. METHODS: RP-HPLC was adopted. The determination was performed on SunFire C18 column with mobile phase consisted of methanol-water (containing triethylamine, pH adjusted to 7.2 using phosphoric acid) (70:30, VIV) at the flow rate of 1.0 ml/min. The detection wavelength was set at 230 nm and column temperature was 30 °C. RESULTS: The linear range of triamcinolone acetonide and diphenhydramine hydrochloride were 0.008-0.048 mg/ml (r= 0.999 0) and 0.048 4-0.290 4 mg/ml (r=0.999 9) with their average recoveries of 99.0% (RSD=1.1%, n=9) and 98.8% (RSD= 1.6%, n=9). CONCLUSIONS: The method is convenient, rapid, accurate. It can be used for quality control of Compound triamcinolone acetonide cream.关键词
复方曲安奈德乳膏/盐酸苯海拉明/反相高效液相色谱法/含量测定Key words
Compound triamcinolone acetonide cream/ Diphenhydramine hydrochloride/ RP-HPLC/ Content determination分类
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陈晓峰,张咏梅,汪明志..RP-HPLC法同时测定复方曲安奈德乳膏中醋酸曲安奈德和盐酸苯海拉明的含量[J].中国药房,2013,24(5):446-447,2.
