中国药房2013，Vol.24Issue(7)：624-627,4.DOI:10.6039/j.issn.1001-0408.2013.07.18
黄连的3D-HPLC特征指纹图谱研究
Study on 3D-HPLC Characteristics Fingerprints of Coptidis Rhizoma
摘要
Abstract
OBJECTIVE: To analyze the alkaloids component of various Coptidis Rhizoma from different producing areas, and to establish 3D-HPLC characteristics fingerprints. METHODS: HPLC-DAD was adopted. The determination was performed on Xti-mate C18(250 mm×4.6 mm,5 μm) column with mobile phase consisted of 30 mmol/L ammonium hydrogen carbonate solution (including 0.1% triethylamine, 0.7% ammonia water)-acetonitrile (gradient elution) at the flow rate of 1.0 ml/min. The detection wavelength was set at 200-400 nm and column temperature was 30 ℃. The similarity evaluation and cluster analysis were carried out to obtained fingerprints of 31 batches of Coptis chinensis, 6 batches of C. deltoidea, 4 batches of C. teeta at 270 nm by similarity evaluation software and SPSS18.0 software. RESULTS: 3D-HPLC-DAD fingerprints of Coptidis Rhizoma were set up to obtain 11 characteristic fingerprint peaks; the common peak area took up over 90% of total peak area. 7 kinds of alkaloids were demarcated by comparing with substance control, such as magnoflorine, jateorrhizine hydrochloride, palmatine hydrochloride, epiberberine hydrochloride, coptisine hydrochloride, palmatine hydrochloride and berberine hydrochloride. Content difference of epiberberine hydrochloride was symbolic feature of 3 kinds of Coptidis Rhizoma. CONCLUSION: The method is accurate, stable and reproducible. The established characteristic fingerprints can be taken as one of main criteria of quality evaluation of Coptidis Rhizoma.关键词
道地药材/黄连/3D-高效液相色谱-二极管阵列检测器法/特征指纹图谱/生物碱Key words
Genunie medicinal materials/ Coptidis Rhizoma/ 3D-HPLC-DAD/ Characteristic fingerprints/ Alkaloid分类
医药卫生引用本文复制引用
岳清洪,唐策,杨永东,范刚,赖先荣,邝婷婷,张艺..黄连的3D-HPLC特征指纹图谱研究[J].中国药房,2013,24(7):624-627,4.基金项目
国家科技支撑计划项目资助(No.2007BAI40B05 （）
No.2007BAI40B03) （）
四川省支撑计划项目资助(No.2008SZ0045) （No.2008SZ0045）
成都中医药大学校基金资助项目(No.ZRZD201103 （）
No.ZRYB201117) （）
