中国医科大学学报2012，Vol.41Issue(11)：965-967,989,4.
创伤后应激障碍大鼠海马分子伴侣葡萄糖调节蛋白94表达的变化
Changes of Molecular Chaperone GRP94 in Hippocampus from the Posttraumatic Stress Disorder Rat Model
摘要
Abstract
Objective To observe the changes of molecular chaperone glucose-regulated protein 94 (GRP94) in the hippocampus of post-traumatic stress disorder rat model. Methods Totally 45 rats were randomly assigned to three groups including a normal control group (nd, n =15) and two SPS groups (sps-7d,n =15;sps-14d,n =15),and prepared for single-prolonged stress (SPS) model. Immunohistochem-istry, Western blot and reverse transcription-polymerase chain reaction (RT-PCR) were performed to examine the changes of GRP94 in the rat hippocampus. Results By using immunohistochemistry and Western blot,the expression of GRP94 in the group of sps-7d was markably increased compared with the normal control group (P < 0.05). The expression of GRP94 in sps-14d group showed a significantly decreased compared to the sps-7d group (P < 0.05). The mRNA expression of GRP94 in RT-PCR had the similar trends of changes. Conclusion SPS would induce changes of GRP94 expression in the rat hippocampus, which provided basis for future study of neuronal apoptosis induced by endoplasmic reticulum stress.关键词
创伤后应激障碍/海马/葡萄糖调节蛋白94/大鼠Key words
posttraumatic stress disorder/hippocampus/glucose-regulated protein 94/rat分类
医药卫生引用本文复制引用
谢菊华,韩芳,石玉秀..创伤后应激障碍大鼠海马分子伴侣葡萄糖调节蛋白94表达的变化[J].中国医科大学学报,2012,41(11):965-967,989,4.基金项目
国家自然科学基金资助项目(81171282) （81171282）
