中国医科大学学报2012，Vol.41Issue(11)：968-971,976,5.
细胞周期蛋白激酶抑制剂flavopiridol对骨肉瘤耐药细胞MG63/ADR增殖抑制的体内外实验研究
Inhibitory Effects of CDKI on MG63/ADR Cells Proliferation both in vitro and in vivo
摘要
Abstract
Objective To investigate the growth-inhibition and apoptosis effects of flavopiridol on human osteosarcoma MG63/ADR cell and the related mechanism both in vitro and in vivo. Methods The proliferation of MG63 and MG63/ADR cells was determined by using MTT method. Cell cycle progression and apoptosis ratio were determined by flow cytometry. The expressions of pro-caspase-9, pro-caspase-3, cas-pase-8,Bcl-2,Bcl-xL,p53,Bax and eleaved-PARP were detected by Western blot. The subcutaneous tumors of MG63/ADR cells were also established in nude mice to study the antitumor effects of flavopiridol. Results After exposure to flavopiriciol, the growth of MG63/ADR cells were inhibited in a time- and dose-dependent manner. Apoptosis rate of MG63/ADR was higher than control group (P<0.05). The ex-pressions of pro-caspase-9, pro-casepase-3, Bcl-2 and Bcl-xL were down-regulated, and the expression of active-form PARP and active-form caspase-8 were up-regulated. The expressions of p53 and Bax were not affected. The in vivo inhibitory effect on ADR clones of osteosarcoma in the treatment group was observed compared with the control group. Conclusion Flavopiridol can inhibit the growth of drug-resistant os-teosarcoma cells both in vitro and vivo. The apoptosis of MG63/ADR cells induced by flavopiridol might via caspase cascade pathway and p53-independent mitochondrial apoptosis pathway.关键词
flavopiridol/细胞周期/骨肉瘤/凋亡/caspaseKey words
flavopiridol/cell cycle/osteosarcoma/apoptosis/caspase分类
医药卫生引用本文复制引用
李旭,李岩,程世孝,李雷明,孟凡贺,朱悦,范广宇..细胞周期蛋白激酶抑制剂flavopiridol对骨肉瘤耐药细胞MG63/ADR增殖抑制的体内外实验研究[J].中国医科大学学报,2012,41(11):968-971,976,5.基金项目
国家自然科学基金资助项目(30973021) （30973021）
