中国医科大学学报2012，Vol.41Issue(11)：990-994,5.
新基因JDP2通过不同通路抑制人胰腺癌上皮向间质转化作用的研究
Effect of JDP2 on Epithelial-mesenchymal Transition in Human Pancreatic Cancer Cell Line
摘要
Abstract
Objective To investigate the relationship of pancreatic cancer epithelial-to-mesenchymal transition(EMT) with the expression of Jun dimerization protein 2 (JDP2). Methods The pancreatic cancer cell line BxPC3 was transfected with JDP2 and induced by Collagen Ⅰ and TGF-β1. Western blot analysis and immunofluorescence were performed to detect the protein expressions of the epithelial marker E-cad-herin and the mesenchymal marker vimentin. Cell migration was determined by Transwell motility assay. Results After EMT induction, the E-cadherin and vimentin expression levels in JDP2 overexpressed cells did not change significantly compared with the non-induced cells (DMSO, negative control), whereas E-cadherin expression decreased and vimentin expression increased in the vector control group cells. Ad-ditionally ,compared with the negative control,only the BxPC3 cells transfected with empty plasmid showed significant morphological changes after Collagen I and TGF-β1 induction (P < 0.05). Conclusion JDP2,as an AP-1-family inhibitor,inhibits EMT,which may lead to a new direction in molecular-targeted therapy for pancreatic cancer.关键词
胰腺癌/转化生长因子β1/胶原蛋白Ⅰ/上皮向间质转化/Jun二聚化蛋白2Key words
pancreatic cancer/TGF-β1/Collgen I/epithelial-to-mesenchymal/Jun dimerization protein 2分类
医药卫生引用本文复制引用
许元鸿,刘哲,郭克建,杜瑞霞,蒋红军..新基因JDP2通过不同通路抑制人胰腺癌上皮向间质转化作用的研究[J].中国医科大学学报,2012,41(11):990-994,5.基金项目
辽宁省科学技术计划项目(2011404013-4) （2011404013-4）
