中国药理学通报2013，Vol.29Issue(1)：107-112,6.DOI:10.3969/j.issn.1001-1978.2013.01.023
知母皂苷元对高糖引起的体外培养大鼠海马神经元损伤的保护作用
Protective effect of Sarsasapogenin against hyperglycemia induced neurotoxicity in cultured hippocampal neurons
摘要
Abstract
Aim To investigate whether Sarsasapoge-nin ( Sar ) can protect hippocampal neurons from hyper-glycemia induced neurotoxicity. Methods Primary cultures were obtained from hippocampi of 0-to 24-h-old Sprague-Dawley rats. After 7 d in culture, the cells were used for the treatment. The cultured neurons were divided into control group, hyperglycemia treatment group, hyperglycemia ( glucose 50 mmol·L -1 ) + Sar ( 10, 30,100 μmol·L-1 )groups. In control group, cultured hippocampal neurons were incubated with 0. 1% DMSO for 48 h. In hyperglycemia treatment group, cultured neurons were incubated with glucose ( 30, 50, 100 mmol·L-1 ) for 48 h. In hyperglycemia + Sar group, Sar was added to the neurons 1 h prior to incubation with glucose 50 mmol·L-1. Cell viability was determined by MTT. Immunostaining and Western blot were used for synaptophysin ( SYP ) protein expression. Neuronal apoptosis was quantified by scoring the percentage of cells with apoptotic nuclear morphology after Hoechst 33258 staining. The cellular extracts were prepared for Western blot of active caspase-3 and poly ADP-ribose polymerase ( PARP ) protein expression. Results Cultured hippocampal neurons treated with hyperglycemia resulted in the decrease in cell viability, the decrease in SYP expression, and the increase in the percentage of apoptotic neurons, active caspase-3 and PARP expressions. Sar prevented the hyperglycemia induced decrease in SYP, and reduced hyperglycemia induced apoptotic morphology and active caspase-3 and PARP protein levels. Conclusion These results indicate that Sar can protect hippocampal neurons against hyperglycemia neurotoxicit. the关键词
知母皂苷元/海马神经元/高糖/突触素/凋亡/caspase-3Key words
Sarsasapogenin/ hippocampal neurons/ hyperglycemia/ synaptophysin/ apoptosis/ caspase-3分类
医药卫生引用本文复制引用
肖复茜,李洪秀,隋海娟,刘卓,屈文慧,郁盛雪,金迎新,金英..知母皂苷元对高糖引起的体外培养大鼠海马神经元损伤的保护作用[J].中国药理学通报,2013,29(1):107-112,6.基金项目
辽宁省教育厅创新团队项目( No LT 2010064) （ No LT 2010064）
辽宁省教育厅一般项目(No L2011143) （No L2011143）
辽宁医学院院内资助课题(No Y2010Z003) （No Y2010Z003）
