中国药理学通报2013，Vol.29Issue(2)：189-193,5.DOI:10.3969/j.issn.1001-1978.2013.02.10
银杏内酯B对内皮细胞的保护作用及分子机制研究
Protective effect of ginkgolide B on ox-LDL stimulated endothelial cells
摘要
Abstract
Aim To investigate the effect of ginkgolide B on ox-LDL stimulated human umbilical vein endothe-lial cells ( HUVECs ). Methods After incubation with ginkgolide B ( 0. 2, 0. 4, 0. 6 g · L-1 ) for 1 h, cells were treated with ox-LDL ( 0. 1 g · L-1 ) for 4 h. The expressions of lectin-like oxidized LDL receptor-1 ( LOX-1 ), Nrf2, PI3K, SIRT1 and Akt phosphoryla-tion were analyzed with Western blot. Results The expression of LOX-1 was increased in ox-LDL-treated HUVECs. In contrast, ginkgolide B significantly decreased the LOX-1 expression. Ginkgolide B attenuated Akt phsphorylation in ox-LDL-treated cells in a dose-dependent manner, but it had no effect on PI3K expression. SIRT1 expression was down-regulated in ox-LDL stimulated cells, whereas ginkgolide B significantly increased SIRT1 expression. Nrf2 expression was up-regulated in cells treated with ox-LDL, and ginkgolide B restored the change of Nrf2 expression significantly. Conclusion Ginkgolide B has protective effects on ox-LDL stimulated endothelial cells, and the mechanism might be associated with reductiion of LOX-1 expression, inhibition of Akt phosphorylation and onset of intracellular protective signaling molecule.关键词
银杏内酯B/人脐静脉内皮细胞/LOX-1/Akt/SIRT1/Nrf2Key words
ginkgolide B/ human umbilical vein endoLhelial cells/ LOX-1 / AkL/ S1RT1 / Nrf2分类
医药卫生引用本文复制引用
马丽娜,陈北冬,赵艳阳,鲍利,吴伟,李兴广,齐若梅..银杏内酯B对内皮细胞的保护作用及分子机制研究[J].中国药理学通报,2013,29(2):189-193,5.基金项目
国家自然科学基金资助项目(No 81070231,No 81270379) （No 81070231,No 81270379）
