中国药理学通报2013，Vol.29Issue(2)：238-241,4.DOI:10.3969/j.issn.1001-1978.2013.02.21
慢性吗啡处理对大鼠交感神经节pCREB和CREB mRNA表达的影响
Effects of chronic morphine treatment on expression of pCREB and CREB mRNA in sympathetic ganglia of rats
摘要
Abstract
Aim To study the effects of chronic morphine treatment on the expression of phosphorylated cAMP response element binding protein( pCREB ) and CREB mRNA in sympathetic ganglia, superior cervical ganglia ( SCG ) of rats. Methods The Wistar rats were randomly divided into 4 groups, namely control, morphine acute, morphine dependent and morphine withdrawal group. The expression of pCREB and CREB mRNA in SCG was detected by means of immu-nohistochemistry and RT-PCR respectively. Results ( 1 ) The content of pCREB in SCG of morphine-acute group was lower than that of control group ( P < 0. 05 ). ( 2 ) The content of pCREB in SCG of morphine dependent group returned to the level of control group with an increasing tendency ( compared with control, P > 0. 05 ; compared with morphine-acute, P<0. 01 ). ( 3 ) Compared with control group, the content of pCREB in SCG of morphine withdrawal group was increased ( P < 0. 01 ). (4) The expressive level of CREB mRNA in SCG was not significantly changed in each group ( P > 0. 05 ). Conclusion There is an up-regulation of pCREB in sympathetic ganglia of chronic morphine treated rats.关键词
吗啡/依赖/耐受/戒断/交感神经节/pCREB/CREB/mRNAKey words
morphine/ dependence/ tolerance/ withdrawal/ sympathetic ganglion/ pCREB/ CREB mRN分类
医药卫生引用本文复制引用
何萍,陈家欢,黄晓亮,于洪波,莫宁..慢性吗啡处理对大鼠交感神经节pCREB和CREB mRNA表达的影响[J].中国药理学通报,2013,29(2):238-241,4.基金项目
广西自然科学基金青年基金资助项目(No 桂科青0542045) （No 桂科青0542045）
广西医科大学博士启动基金资助项目(No 308054) （No 308054）
