中国药理学通报2013，Vol.29Issue(2)：261-265,5.DOI:10.3969/j.issn.1001-1978.2013.02.26
毛茛有效部位D1对人胃癌细胞MGC803血管生成拟态的作用及其机制
Effect of ranunculus japonicus components D1 on vasculogenic mimicry of human gastric cancer cell MGC803
摘要
Abstract
Aim To study the effect of ranunculus ja-ponicus components Dl on vasculogenic mimicry of human gastric cancer cell MGC803 in vitro and its possible mechanisms. Methods The effect of Dl on the cell adhesion of human gastric cancer cell MGC803 was observed by cell attachment assay. The effect of Dl on the cell migration of MGC803 was observed by cell wound healing assay. The function of Dl on the tube-like structures formation in human gastric cancer cell MGC803 in vitro were observed by tube formation assay. The effect of Dl on the expression of vasculogenic mimicry related genes was assessed by semi-quantitative RT-PCR assay. Results Ranunculus japonicus components Dl effectively inhibited human gastric cancer cell MGC803 cell adhesion, cell migration and cancer cell mediated vasculogenic mimicry in vitro in a dosage-dependent manner. Furthermore, the semi-quantitative RT-PCR showed that Dl markedly inhibited the expression of vasculogenic mimicry related genes VE-Cadherin, Sema 4D, MMP2 and Integrin (35. Conclusion Ranunculus japonicus components Dl can effectively inhibit human gastric cancer cell MGC803 cell attachment, cell migration and cancer cell mediated vasculogenic mimicry by down regulating vasculogenic mimicry related genes VE-Cadherin, Sema 4D, MMP2 and Integrin (35. The study suggests that Dl is a promising candidate of anti-gastric cancer mediated vasculogenic mimicry.关键词
毛茛/胃癌/血管生成拟态/MGC803/细胞粘附/细胞迁移Key words
ranunculus japonicus/ gastric cancer/ vasculogenic mimicry/ MGC803 / cell attachment/ cell migration分类
医药卫生引用本文复制引用
虞迪,朱峰妍,曹志飞,王薇丹,鲍美美,顾振纶,周泉生..毛茛有效部位D1对人胃癌细胞MGC803血管生成拟态的作用及其机制[J].中国药理学通报,2013,29(2):261-265,5.基金项目
苏州市科技支撑计划项目(No SS201004). （No SS201004）
