中国药理学通报2013，Vol.29Issue(2)：290-293,4.DOI:10.3969/j.issn.1001-1978.2013.02.32
SD大鼠寒积里实证模型的建立与评价
Establishment and evaluation of SD rats model with cold accumulation and internal excess
摘要
Abstract
Aim To establish and evaluate Sprague-Dawley ( SD ) rats model with cold accumulation and internal excess under the guidance of traditional Chinese medicine theories. Methods Rats model with cold accumulation and internal excess was induced by oral administration of ice water firstly, then kaolin. Changes of the biological characteristics of the model animals, the excretion, tail temperature measurement and the detection of intestinal neurotransmitters, gastrointestinal hormone, cAMP, cGMP and other objective indicators were observed. Results The general biological characteristics of the model animal performance were performed as: puffed and withered hair, gathering at one end in cages, dry stools and reduced food consumption. Compared with the control group, defecation grains of model group reduced significantly within six hours. Meanwhile tail temperature reduced significantly, and motilin, vasoactive intestinal peptide, substance P secretion were in disorder and cAMP/ cGMP reduced. After being treated with Dahuang Fuzi Tang, symptoms and indicators were improved markedly. Conclusion SD rats model with cold accumulation and internal excess can be stably established by administration of ice water firstly, then kaolin.关键词
寒积里实/SD大鼠/模型/建立/大黄附子汤/评价Key words
cold accumulation and internal excess/ SD rats/ model/ establishment/ Dahuang Fuzi Tang/ evaluation分类
医药卫生引用本文复制引用
李嬛,吴丽,李杰,郭辉,刘晓,蔡皓,蔡宝昌..SD大鼠寒积里实证模型的建立与评价[J].中国药理学通报,2013,29(2):290-293,4.基金项目
国家自然科学基金资助项目(No 81073022) （No 81073022）
