中国药理学与毒理学杂志2013，Vol.27Issue(1)：54-60,7.DOI:10.3867/j.issn.1000-3002.2013.01.011
丹皮酚体外对肝星状细胞增殖和凋亡的影响
Effect of paeonol on proliferation and apoptosis of hepatic stellate cells in vitro
摘要
Abstract
OBJECTIVE To study the effect of paeonol on cell proliferation and apoptosis of hepatic stellate cells (HSC). METHODS HSC were treated with paeonol 1.66 -49.8 mg · L-1 for 48 h, and the viability of HSC was detected with MTT assay and trypan blue staining. The viability of HSC was also determined using trypan blue staining after treatment with paeonol 33.2 mg·L-1 for 6, 12, 24, 36 and 48 h, respectively. Cytotoxicity of paeonol was determined with lactate dehydrogenase (LDH) release assay. Leica microscope and Hoechst 33258 dyeing were used to observe morphological changes of HSC. Flow cytometry analysis was used to detect the apoptosis rate of HSC and cell cycle. RESULTS MTT assay results showed that paeonol 16.6 -49.8 mg·L-1 had significant inhibitory effect on HSC proliferation in a concentration-dependent manner (r=0.955, P<0.05), the IC50 value was 105.4 mg·L-1 after intervention for 48 h. Trypan blue staining results showed that paeonol not only reduced the survival rate of HSC in a concentration-dependent relationship (r=0.936, P<0.05), but also in a time-dependent manner (r=0.965, P<0.05) at the concentration of 33.2 mg·L-1. Paeonol 1.66 -69. 7 mg·L-1 had no obvious cytotoxic effect on HSC.Flow cytometry assay results showed that paeonol 8.3 - 49.8 mg·L-1 significantly increased HSC apoptosis (P<0.05, P<0.01) in a concentration-dependent manner (r=0.920, P<0.05). Paeonol 33.2 and 49.8 mg·L-1 arrested HSC cell cycle at G0/G1 phase (P<0.01), and reduced the percentage of S phase cells (P<0.01) also in a concentration-dependent manner (r=0.980, P<0.05). CONCLUSION Paeonol can inhibit HSC proliferation and induce apoptosis, which may underlie the antifibrotic effect of paeonol.关键词
丹皮酚/肝纤维化/肝星状细胞/凋亡/增殖Key words
paeonol/ hepatic fibrosis/ hepatic stellate cell/ apoptosis/ proliferation分类
医药卫生引用本文复制引用
魏东华,孔德松,张自力,倪春艳,张雪娇,郑仕中..丹皮酚体外对肝星状细胞增殖和凋亡的影响[J].中国药理学与毒理学杂志,2013,27(1):54-60,7.基金项目
国家自然科学基金(81270514) （81270514）
国家自然科学基金(30873424) （30873424）
十一五科技支撑计划(2008BAI51B02) （2008BAI51B02）
教育部博士点博导基金(20103237110010) （20103237110010）
江苏省自然科学基金(BK2008456) （BK2008456）
江苏省六大人才基金(2009-B-010) （2009-B-010）
江苏省针灸学重点实验室开放课题(KJA200801) （KJA200801）
江苏高校优势学科建设工程资助项目PAPD(ysxk-2010) （ysxk-2010）
南京中医药大学中药学一级学科开放课题资助(2011ZYX4-008) （2011ZYX4-008）
