中国油料作物学报2012，Vol.34Issue(5)：537-543,7.
基于磁性固定化酶的α-亚麻酸植物甾醇酯合成工艺研究
Immobilization of Candida rugosa lipase on magnetic polymer microsphere for synthesis of phytosterol esters of alpha-linolienic acid
摘要
Abstract
Immobilized enzyme of Candida rugosa lipase on magnetic microsphere was used as biocatalyst for synthesis of phytosterol esters of alpha - linolienic. Response surface methodology ( RSM) was employed to evaluate the effects of synthesis conditions. The optimum synthesis conditions were as follows; 25. 2mg/mL immobilized CRL, 146. 5μmol/mL phytosterols concentration, at 55℃ for 5h in the presence of isooctane, the degree of phytos-terols esterification was 92. 61%. By solvent cleaning, the immobilization CRL sustained 78. 1% activities after having been recycled 10 times.关键词
磁性颗粒/酯化反应/固定化脂肪酶/甾醇酯Key words
Magnetic microsphere/ Esterification/ Immobilization lipase/ Phytosterol esters分类
化学化工引用本文复制引用
董玲,郑明明,郭萍梅,李文林,邓乾春,黄凤洪,郑畅..基于磁性固定化酶的α-亚麻酸植物甾醇酯合成工艺研究[J].中国油料作物学报,2012,34(5):537-543,7.基金项目
国家自然科学基金(31101353,31000777) （31101353,31000777）
十二五农村领域国家科技计划(2011BAD22B04) （2011BAD22B04）
现代农业产业技术体系建设专项(CARS-13) （CARS-13）
中国农业科学院油料作物研究所所长基金(1610172011014) （1610172011014）
