中国油料作物学报2012，Vol.34Issue(6)：567-574,581,9.
甘蓝型油菜中KCS6基因的克隆和功能分析
Cloning and functional characterization of KCS6 genes from Brassica napus
摘要
Abstract
GenomeWalking together with RACE techniques were used to clone KCS6 genes from B. napus cv. Zhongshuang 9. Two copies of KCS6 gene were found in B. napus, depending on their ancestral origin. One was BnKCS6c, showing 99% identity to corresponding B. oleracea mRNA. The other was BnKCSda showing 99% identity to corresponding B. rapa mRNA. Both genes had a 1 494bp - coding region composed by 2 exons spanning 1 045bp and 449bp respectively. In early stage of seed development, KCS6 expression level was high in silique shell and seeds, but rapidly reduced when seeds matured. Both transgenic KCS6 genes could be normally translated into proteins in yeast cells, but no synthesis of very long chain fatty acids was observed. It indicated that the heter-ologous expressed KCS6 protein has no catalytic activity,or KCS6 protein is not able to utilize the yeast fatty acid as catalytic substrate to synthesize very long chain fatty acid.关键词
甘蓝型油菜/KCS6基因/克隆/表达谱/转基因/酵母表达Key words
Brassica napus/ KCS6 gene/ Cloning/ Expression pattern/ Transgene/ Heterologous expression in yeast cell分类
农业科技引用本文复制引用
武玉花,曹应龙,罗军玲,邢素娜,吴磊,李晓飞,卢长明..甘蓝型油菜中KCS6基因的克隆和功能分析[J].中国油料作物学报,2012,34(6):567-574,581,9.基金项目
国家自然科学基金(31100190) （31100190）
