中国油料作物学报2012，Vol.34Issue(6)：582-585,4.
抗草甘膦基因mEPSPS转化油菜研究
Genetic transformation of rapeseed with the glyphosate-resistant gene mEPSPS
摘要
Abstract
Previously cloned mEPSPS gene [conferring glyphosate herbicide (Roundup) , resistance] was introduced into a Brassica napus line Jia 572 by Agrobacterium - mediated transformation method. Molecular analysis demonstrated that 4 independent transgenic plants were obtained, and the mEPSPS gene was inherited to the next generation. RT - PCR analysis indicated that the mEPSPS gene could be successfully transcribed in transgenic rapeseed plants although the expression levels varied among different lines. Herbicide resistance assay by Roundup spray showed that the transgenic plants survived under 100 - time diluted solution with commercial glyphosate -containing 41% herbicide (as 3 039mg/L glyphosate) , while the wild type died even under a 200 -time diluted solution.关键词
油菜/mEPSPS基因/转基因/RT-PCR表达分析/农达/抗除草剂Key words
Rapeseed/mEPSPS gene/ Transgene/ RT - PCR analysis/ Roundup/Herbicide resistance分类
生物科学引用本文复制引用
柳寒,周永明..抗草甘膦基因mEPSPS转化油菜研究[J].中国油料作物学报,2012,34(6):582-585,4.基金项目
国家油菜产业技术体系(nycytx-00503) （nycytx-00503）
