中国医学装备2012，Vol.9Issue(12)：43-45,3.
溶血对电化学发光免疫法测定胰岛素和C-肽的影响
Influence of hemolysis on detection of insulin and C-peptide using electrochemiluminescence immunoassay
郑慕阳 1陈海斌 2梁业斌2
作者信息
- 1. 梧州市中医医院检验科 广西梧州543002
- 2. 梧州市人民医院检验科 广西梧州543001
- 折叠
摘要
Abstract
Objective: To approach specimen hemolysis degree and hemolytic specimen waiting period interfering with the testing results while using electrochemiluminescence immunoassay (ECLIA) to test serum insulin and C-peptide. Methods: We artificially interfered with the test specimen into 5 hemolysis specimens with different hemolysis degrees. And we placed them in indoor environment (20℃). Using ECLIA to test and analyze insulin and C-peptide degree
of hemolytic and non-hemolytic specimens separately during 4 different time quanta (0, 30, 60, 120 min). Results: Specimen hemolysis can lower insulin concentration. Insulin concentration testing and specimen hemolytic degree and waiting period are in negative correlation. Specimen hemolysis has no influence on using Electrochemiluminescence Immunoassay to test C-peptide. Conclusion: Definitely avoid hemolysis during insulin test. Under the circumstance of unsure quality control before specimen analysis, C-peptide releasing horizontal curve is more reliable in clinical practices than insulin releasing horizontal curve.关键词
溶血/电化学发光免疫法/胰岛素/C-肽Key words
Hemolysis/ Electrochemiluminescence Immunoassay/ Insulin/ C-peptide分类
医药卫生引用本文复制引用
郑慕阳,陈海斌,梁业斌..溶血对电化学发光免疫法测定胰岛素和C-肽的影响[J].中国医学装备,2012,9(12):43-45,3.
