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高效液相色谱法测定维吾尔药复方赛比尔片中芦荟苷含量

沙吉旦阿不来提 尼加提阿不来提 热孜万阿不都热依木

中国药业2013,Vol.22Issue(2):22-23,2.
中国药业2013,Vol.22Issue(2):22-23,2.

高效液相色谱法测定维吾尔药复方赛比尔片中芦荟苷含量

Determination of Aloe Content in Uighur Medicine Compound Saibier Tablet by HPLC

沙吉旦阿不来提 1尼加提阿不来提 1热孜万阿不都热依木1

作者信息

  • 1. 新疆维吾尔自治区喀什地区药品检验所,新疆,喀什,844000
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摘要

Abstract

Objective To establish a HPLC method for determining the aloe content in Uighur medicine Compound Saibier Tablet. Methods The high performance liquid chromatographyv HPLC ) was adopted with the Pheomenex lima C18 column! 250 mm x 4.60 mm,5 μm) as the chromatography column,the temperature of 35℃,the mobile phase of acetonitrile - water (25 : 75), the detection wavelength of 355 nm and the flow velocity of 1.0 mL/min. Results The mass concentration of aloe in the range of 10.54-210.80 μg/mL showed good linear relationship with the peak area, r = 0. 999 5. The average recovery rate was 97. 53% , RSD 1.27%. Conclusion This method has the advantages of simple operation, sensitivity, accuracy and remarkably isolated effect, and can be used for the determination of the aloe content and the quality control of this product.

关键词

复方赛比尔片/高效液相色谱法/芦荟苷/含量测定

Key words

Compound Saibier Tablet/HPLC/aloe/ content determination

分类

医药卫生

引用本文复制引用

沙吉旦阿不来提,尼加提阿不来提,热孜万阿不都热依木..高效液相色谱法测定维吾尔药复方赛比尔片中芦荟苷含量[J].中国药业,2013,22(2):22-23,2.

中国药业

OACSTPCD

1006-4931

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